Identification of photosystem I components from the cyanobacterium, Synechococcus vulcanus by N‐terminal sequencing
Identification of photosystem I components from the cyanobacterium, Synechococcus vulcanus by N‐terminal sequencing
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通过 N 端测序鉴定蓝藻、聚球藻的光系统 I 组分
DOI：
10.1016/0014-5793(89)80971-8
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发表时间：
1989
期刊：
影响因子：
3.5
通讯作者：
Y. Inoue
中科院分区：
文献类型：
--
作者：
H. Koike;M. Ikeuchi;T. Hiyama;Y. Inoue
从嗜热蓝细菌，硫化聚球藻，分离的光系统I核心复合物，是由8个低分子量的蛋白质的18，14，12，9.5，9，6.5，5和4.1 kDa的除了PS I叶绿素蛋白。测定了所有这些组分的N-末端氨基酸序列，并与高等植物进行了比较。显然，9.5 kDa组分对应于携带非血红素铁-硫中心A和B的蛋白质。这种蛋白质通过染色很难显现，因此在凝胶电泳分析中可能被忽略。18、14、12和9 kDa组分与高等植物PS I的相应亚基显示出明显的同源性。相反，6.5、5和4.1kDa组分不对应于任何已知蛋白质，除了4.1kDa组分的序列与叶绿体DNA的未鉴定的开放阅读框（ORF）42（苔类植物）或ORF 44（烟草）相匹配。
The photosystem I core complex isolated from a thermophilic cyanobacterium,Synechococcus vulcanus, is composed of eight low-molecular-mass proteins of 18, 14, 12, 9.5, 9, 6.5, 5 and 4.1 kDa in addition to the PS I chlorophyll protein. N-terminal amino acid sequences of all these components were determined and compared with those of higher plants. Clearly, the 9.5 kDa component corresponds to the protein which carries the non-heme iron-sulfur centers A and B. This protein is so poorly visualized by staining that it has probably been overlooked in gel electrophoresis analyses. The 18, 14, 12 and 9 kDa components show appreciable homology with respective subunits of higher plant PS I. In contrast, the 6.5, 5 and 4.1 kDa components do not correspond to any known proteins except that the sequence of the 4.1 kDa component matches an unidentified open reading frame (ORF) 42 (liverwort) or ORF44 (tobacco) of chloroplast DNA.
完整的 P-700 和包含 Fx 的光系统 I 核心蛋白的纯化和特性。
DOI：
10.1016/s0005-2728(89)80439-6
发表时间：
1989
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Parrett,KG;Mehari,T;Warren,PG;Golbeck,JH
通讯作者：
Golbeck,JH