Identification of homologues to the human cytomegalovirus US22 gene family in human herpesvirus 6.
Identification of homologues to the human cytomegalovirus US22 gene family in human herpesvirus 6.
复制标题
人类疱疹病毒 6 中人类巨细胞病毒 US22 基因家族同源物的鉴定。
DOI：
--
复制
发表时间：
1992
影响因子：
3.8
通讯作者：
B. Barrell
中科院分区：
文献类型：
--
作者：
S. Efstathiou;G. Lawrence;Chris M. Brown;B. Barrell
测定了人疱疹病毒6型（HHV-6）U1102株基因组Sal Ⅰ H和Sma Ⅰ G重叠限制性片段的10079 bp序列。该序列含有6个完整的开放阅读框（ORF）和2个不完整的ORF，分别位于SalI H和SmaI G片段的5 '和3'端。这些ORF中的7个仅在β-疱疹病毒人类巨细胞病毒（HCMV）中具有可识别的同源物，在人类α-疱疹病毒、水痘-带状疱疹病毒和单纯疱疹病毒1型或γ-疱疹病毒Epstein-Barr病毒的基因组中没有明显的对应物。测序的DNA位于HHV-6基因组的左侧重复序列附近，在包含保守疱疹病毒基因块的公认区域之外。本研究中鉴定的HHV-6 ORF与HCMV中的对应物ORF UL23、UL24和UL27至UL31之间明显存在密切的共线性关系。HHV-6 ORF中的四个SHL1、SHL2、SFL1和SSL2与HCMV US22蛋白家族的成员相关，其自身串联排列并且主要位于原型HCMV株AD 169基因组的独特长区域的独特短和左端内。两个相邻的HHV-6 ORF，SSL1和SHL3，与HCMV UL27相关。除了HHV-6 ORF与HCMV US22家族的氨基酸序列相似性之外，该基因集的鉴定表明这两种人类疱疹病毒之间特别密切的关系，并表明这些相关串联排列的基因的聚类可能是β-疱疹病毒型基因组的一般特征。
The sequence of 10079 bp corresponding to the overlapping SalI H and SmaI G restriction fragments of the genome of human herpesvirus 6 (HHV-6) strain U1102 was determined. The sequence contains six complete open reading frames (ORFs) and two incomplete ORFs located at the 5' and 3' ends of the SalI H and SmaI G fragments respectively. Seven of these ORFs have recognizable homologues only in the beta-herpesvirus human cytomegalovirus (HCMV), no obvious counterparts being detectable in the genomes of the human alpha-herpesviruses, varicella-zoster virus and herpes simplex virus type 1 or the gamma-herpesvirus Epstein-Barr virus. The DNA sequenced is located proximal to the left repeat of the HHV-6 genome outside the well recognized region encompassing conserved herpesvirus gene blocks. A close colinear relationship is evident between the HHV-6 ORFs identified in this study and their counterparts in HCMV, ORFs UL23, UL24 and UL27 to UL31. Four of the HHV-6 ORFs, SHL1, SHL2, SFL1 and SSL2, are related to members of the HCMV US22 family of proteins, which are themselves tandemly arranged and located predominantly within the unique short and the left end of the unique long region of the prototype HCMV strain AD169 genome. Two adjacent HHV-6 ORFs, SSL1 and SHL3, are related to HCMV UL27. The identification of this gene set in addition to the HHV-6 ORFs with amino acid sequence similarity to the HCMV US22 family indicates a particularly close relationship between these two human herpesviruses, and suggests that the clustering of these related tandemly arranged genes may be a general feature of beta-herpesvirus-type genomes.