Purification and characterization of thymol blue for spectrophotometric pH measurements in rivers, estuaries, and oceans
Purification and characterization of thymol blue for spectrophotometric pH measurements in rivers, estuaries, and oceans
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DOI：
10.1016/j.aca.2019.09.009
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发表时间：
2019-12-20
影响因子：
6.2
通讯作者：
Byrne, Robert H.
中科院分区：
文献类型：
--
作者：
Hudson-Heck, Ellie;Byrne, Robert H.
百里酚蓝 (TB) 是一套适用于分光光度法测定水溶液 pH 值的指示剂染料之一。对于海水 pH 值的测量，最常使用间甲酚紫 (mCP)，但 TB 特别适合在表层或浅水区进行测量，其中 pH 值可能超出 mCP 的最佳指示范围（例如，由于光合作用）。这项工作提出了用于纯化市售 TB 的快速色谱程序，并描述了纯化染料的物理化学特性，从而能够在广泛的实际盐度 (S-P) 和温度 (T) 范围内获取分光光度 pH 测量值。 0 的结核病基本特征
Thymol blue (TB) is one of a suite of indicator dyes appropriate for spectrophotometric determinations of the pH of aqueous solutions. For measurements of seawater pH, meta-cresol purple (mCP) is most often used, but TB is especially well suited for measurements in surface or shallow waters where the pH may exceed the optimal indicating range of mCP (e.g., due to photosynthesis). This work presents flash chromatography procedures for purifying commercially available TB and describes physical-chemical characteristics of the purified dye, thus enabling the acquisition of spectrophotometric pH measurements over a wide range of practical salinities (S-P) and temperatures (T). The essential TB characteristics for 0