Biogeography and phenotypic variation in Trichoderma sect. Longibrachiatum and associated Hypocrea species
Biogeography and phenotypic variation in Trichoderma sect. Longibrachiatum and associated Hypocrea species
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DOI：
10.1017/s0953756296002845
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发表时间：
1997-04
期刊：
影响因子：
2.5
通讯作者：
D. Turner;W. Kovacs;K. Kuhls;E. Lieckfeldt;B. Peter;I. Arisan-Atac;J. Strauss;G. Samuels;T. B
中科院分区：
文献类型：
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作者：
D. Turner;W. Kovacs;K. Kuhls;E. Lieckfeldt;B. Peter;I. Arisan-Atac;J. Strauss;G. Samuels;T. B
利用指纹图谱（M13、[GTG]5、[GACA]4）和随机引物（V5），对145株长尾木霉组和长尾木霉属（Hypocrea）菌株进行了随机扩增多态性DNA （RAPD）分析，并对长尾木霉组命名种进行了鉴定。为此，对长尾盘尾绦虫、拟柯宁绦虫、黄芽绦虫、parceramosum和reesei的前型菌株进行了分析，并利用RAPD特征相似性系数作为菌株与单个物种比对的标准。saturnisporum和T. ghanense的前型菌株与长尾木霉组的其他前型菌株表现出相同的种间相似性指数，并且在长尾木霉组中聚集良好，因此被纳入本节。103株分离物鉴定为长尾木霉组成员，并进行进一步研究。大部分变形菌株可与黄绿T.或长尾链T.相对应，而饱和T.和parceramosum T.则相对较少。未发现与前型T. reesei和pseudokoningii具有同源性的自然变异。虽然长尾霉和黄绿霉在其地理分布范围上有很多重叠，但前者在非洲和印度存在，而在东南亚不存在，而后者则相反。非洲型长腕表绦虫与前型长腕表绦虫、长腕表绦虫和saturnisporum绦虫的距离相等。该菌株属于这一类群。pseudokoningii是schweinitzii的变种，仅在澳大利亚东部和新西兰发现。试图鉴定其他变形的潜在远形，发现了另一个发生在欧洲和美国温带气候的施韦尼茨人亚群，与T. citrinoviride同源。没有一株下丘脑菌可以被鉴定为长臂状体、parceramosum和saturnisporum的远形体。
Random amplification of polymorphic DNA (RAPD), using both fingerprinting (M13, [GTG]5, [GACA]4) and random [V5] primers, has been used to analyse 145 isolates characteristic of Trichoderma sect. Longibrachiatum and Hypocrea species with anamorphs referable to that section, and to identify strains with named species of sect. Longibrachiatum. To this end, ex-type strains of T. longibrachiatum, T. pseudokoningii, T. citrinoviride, T. parceramosum and T. reesei were analysed, and the similarity coefficients of RAPD characters used as a criterion for the alignment of strains to individual species. The ex-type strains of T. saturnisporum and T. ghanense exhibited the same interspecific similarity index as the other ex-type strains of Trichoderma sect. Longibrachiatum, and clustered well within it and were therefore included into this section. 103 isolates were identified as members of Trichoderma sect. Longibrachiatum and further investigated. Most of the anamorphic isolates could be aligned with T. citrinoviride or T. longibrachiatum, whereas only a few strains of T. saturnisporum and T. parceramosum were found. No naturally occurring anamorphs with homology to the ex-type strains of T. reesei and T. pseudokoningii were found. While T. longibrachiatum and T. citrinoviride overlapped through much of their geographic ranges, the former was present in Africa and India but not in southeast Asia, while the reverse was true of the latter. The African strains of T. longibrachiatum were equally distant from the ex-type strains of T. longibrachiatum, T. parceramosum and T. saturnisporum. The type strain of T. ghanense was identified as belonging to this group. T. pseudokoningii, the anamorph of Hypocrea schweinitzii, was found exclusively in eastern Australia and New Zealand. Attempts to identify potential teleomorphs of the other anamorphs revealed another subgroup of H. schweinitzii, occurring in temperate climates in Europe and U.S.A., as homologous to T. citrinoviride. No Hypocrea strains could be identified as teleomorphs of T. longibrachiatum, T. parceramosum or T. saturnisporum.