Baicalein-Enriched Fraction Extracted from Oroxylum indicum (L.) Benth. ex Kurz Leaves Exerts Antioxidant and Inhibitory Effects Against Glioblastoma Multiforme
Baicalein-Enriched Fraction Extracted from Oroxylum indicum (L.) Benth. ex Kurz Leaves Exerts Antioxidant and Inhibitory Effects Against Glioblastoma Multiforme
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DOI：
10.3390/pr7120963
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发表时间：
2019-12-01
期刊：
影响因子：
3.5
通讯作者：
Tan, Suat Cheng
中科院分区：
文献类型：
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作者：
Kang, In Nee;Salleh, Nik Nur Hakimah Nik;Tan, Suat Cheng
多形性胶质母细胞瘤（GBM）是原发性脑癌中最恶性的亚型。迄今为止，GBM 的标准临床治疗效果有限，并且可能会产生额外的副作用。最近，从天然植物中分离出的许多生物活性化合物似乎具有有益的抗癌特性。在此，黄芩素（一种从 Oroxylum indicum (L.) Benth 中提取的生物活性黄酮类化合物）对 GBM 的抑制作用。前库尔兹，进行了评估。首先，使用三种溶剂提取黄芩素。我们发现，与仅使用甲醇 (13%) 或水溶液 (0.04%) 的单提取系统相比，使用石油醚和甲醇 (PM) 组合的二元提取系统产生的黄芩素含量最高 (15%)。为了进一步提高PM粗提物中黄芩素的得率，对其进行了富集分馏程序，成功地将黄芩素从最初的粗提物（15%）提高到了富集馏分5（F5）（29%）近两倍。与粗提物相比，富集的 F5 不仅表现出显着更高（约 2.5 倍）的抗氧化特性，而且还发现它能显着抑制 GBM 细胞增殖，比粗提物好约 2.5 倍。总之，本研究成功地优化了提取程序，提高了野花叶中黄芩素代谢物的产量，并增强了其治疗 GBM 的潜力。
Glioblastoma multiforme (GBM) is the most malignant subtype of primary brain cancer. To date, standard clinical treatment for GBM is limited in effectiveness and could impose additional side effects. Recently, numerous bioactive compounds isolated from natural plants appear to have beneficial anti-cancer properties. Here, the GBM inhibitory effect of baicalein, a bioactive flavonoid extracted from Oroxylum indicum (L.) Benth. ex Kurz, was evaluated. Firstly, three solvents were used to extract the baicalein. We found that the binary extraction system, using a combination of petroleum ether and methanol (PM), yielded the highest amount of baicalein (15%) compared to the mono extraction system using methanol (13%) or aqueous (0.04%) only. In order to further enhance the baicalein yield in PM crude extract, it was subjected to an enrichment fractionation procedure, which successfully increased the baicalein by nearly two-fold from the initial crude extract (15%) to the enriched fraction 5 (F5) (29%). The enriched F5 not only showed significantly higher (similar to 2.5-fold) antioxidant properties as compared to the crude extract, it was also found to significantly suppress GBM cell proliferation similar to 2.5-fold better than the crude extract. In conclusion, this study successfully optimized an extraction procedure for increased yield of baicalein metabolite from O. indicum leaves and enhanced its therapeutic potential for GBM treatment.