UPTAKE OF METHYLMERCURY IN THE RAT-BRAIN - EFFECTS OF AMINO-ACIDS
UPTAKE OF METHYLMERCURY IN THE RAT-BRAIN - EFFECTS OF AMINO-ACIDS
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DOI：
10.1016/0006-8993(88)90581-1
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发表时间：
1988-10-11
期刊：
影响因子：
2.9
通讯作者：
CLARKSON, TW
中科院分区：
文献类型：
--
作者：
ASCHNER, M;CLARKSON, TW
Aspects of the regulation of methylmercury (MeHg) transport across the blood-brain barrier (BBB) were investigated in the in vivo Long-Evans female rat. Fifteen s after intracarotid injection, brain203Hg concentration was significantly increased in animals injected with 0.05 mM [203Hg]MeHgCl plus 0.1 mMl-cysteine compared with controls (P< 0.05). Thisl-cysteine-enhanced203Hg brain uptake was abolished by coinjections of [203Hg]MeHgCl with 0.1 mMl-cysteine-l-methionine, or 0.1 mMl-cysteine plus AT-125 (α S, 5S-α-amino-3-chloro-4,5-dihydro-5-isoxazolacetic acid), an irreversible inhibitor of γ-glutamyl transpeptidase. Coinjections of rats with [203Hg]MeHgCl and 0.1 mMd-cysteine did not result in an increase in the rate of203Hg uptake compared with controls. Furthermore, [203Hg]MeHg uptake at 15 s after intracarotid injections in the rat was stereospecific to the neutral amino acid carrier, as203Hg uptake across the BBB was not inhibited by coinjections of [203Hg]MeHgCl with aspartic acid, an acidic amino acid. These results indicate the presence in brain capillaries of a transport system capable of selectively mediating MeHg uptake across the brain capillary endothelial cell membrane.
