Diverse diazotrophs are present on sinking particles in the North Pacific Subtropical Gyre.
Diverse diazotrophs are present on sinking particles in the North Pacific Subtropical Gyre.
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DOI：
10.1038/s41396-018-0259-x
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发表时间：
2019-01
期刊：
影响因子：
--
通讯作者：
Zehr JP
中科院分区：
文献类型：
--
作者：
Farnelid H;Turk-Kubo K;Ploug H;Ossolinski JE;Collins JR;Van Mooy BAS;Zehr JP
下沉颗粒将碳和营养物质从表层海洋运输到深海，被认为是细菌多样性和活动的热点。在贫营养海洋中，固氮生物（固氮生物）是新氮的重要来源，但这些生物的存在和下沉颗粒的输出程度尚不清楚。沉降颗粒收集每6小时超过2天的时间内，在北太平洋副热带环流150米部署在网陷阱。使用16 S rRNA和nifH基因扩增子测序的细菌群落和组合物的固氮菌与个人和散装下沉颗粒进行了评估。散装颗粒中的细菌群落组成在整个时间和空间中保持显著一致，而单独挑选的颗粒观察到较大的变化。这种差异表明，独特的生物地球化学条件下，个别颗粒可能提供不同的生态位专门的细菌类群。与周围的海水相比，颗粒样品富集在不同大小的类的全球显着的N2固定蓝藻，包括束丝藻，硅藻的共生体，和单细胞蓝藻Crocosphaera和UCYN-A。颗粒还包含不同的非蓝藻固氮菌的nifH基因序列，这表明颗粒可能是异养细菌固氮的位点。结果表明，颗粒物上存在多种固氮菌，因此新的N可能直接从表层水的下沉颗粒沃茨中输出。
Sinking particles transport carbon and nutrients from the surface ocean into the deep sea and are considered hot spots for bacterial diversity and activity. In the oligotrophic oceans, nitrogen (N2)-fixing organisms (diazotrophs) are an important source of new N but the extent to which these organisms are present and exported on sinking particles is not well known. Sinking particles were collected every 6 h over a 2-day period using net traps deployed at 150 m in the North Pacific Subtropical Gyre. The bacterial community and composition of diazotrophs associated with individual and bulk sinking particles was assessed using 16S rRNA and nifH gene amplicon sequencing. The bacterial community composition in bulk particles remained remarkably consistent throughout time and space while large variations of individually picked particles were observed. This difference suggests that unique biogeochemical conditions within individual particles may offer distinct ecological niches for specialized bacterial taxa. Compared to surrounding seawater, particle samples were enriched in different size classes of globally significant N2-fixing cyanobacteria including Trichodesmium, symbionts of diatoms, and the unicellular cyanobacteria Crocosphaera and UCYN-A. The particles also contained nifH gene sequences of diverse non-cyanobacterial diazotrophs suggesting that particles could be loci for N2 fixation by heterotrophic bacteria. The results demonstrate that diverse diazotrophs were present on particles and that new N may thereby be directly exported from surface waters on sinking particles.
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影响因子：
3.3
作者：
Frank, Ildiko E.;Turk-Kubo, Kendra A.;Zehr, Jonathan P.
通讯作者：
Zehr, Jonathan P.
影响因子：
4.9
作者：
Berthelot, H.;Moutin, T.;Bonnet, S.
通讯作者：
Bonnet, S.
影响因子：
2.5
作者：
Carpenter, EJ;Montoya, JP;Capone, DG
通讯作者：
Capone, DG
DOI：
10.1038/ismej.2011.26
发表时间：
2011-09
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
64.8
作者：
Guidi L;Chaffron S;Bittner L;Eveillard D;Larhlimi A;Roux S;Darzi Y;Audic S;Berline L;Brum J;Coelho LP;Espinoza JCI;Malviya S;Sunagawa S;Dimier C;Kandels-Lewis S;Picheral M;Poulain J;Searson S;Tara Oceans coordinators;Stemmann L;Not F;Hingamp P;Speich S;Follows M;Karp-Boss L;Boss E;Ogata H;Pesant S;Weissenbach J;Wincker P;Acinas SG;Bork P;de Vargas C;Iudicone D;Sullivan MB;Raes J;Karsenti E;Bowler C;Gorsky G
通讯作者：
Gorsky G