Swine TRIM21 restricts FMDV infection via an intracellular neutralization mechanism
Swine TRIM21 restricts FMDV infection via an intracellular neutralization mechanism
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DOI：
10.1016/j.antiviral.2016.01.004
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发表时间：
2016-03-01
影响因子：
7.6
通讯作者：
Li, Xiangmin
中科院分区：
文献类型：
--
作者：
Fan, Wenchun;Zhang, Dong;Li, Xiangmin
TRIM 21是一种广泛表达的E3泛素连接酶和细胞内抗体受体。TRIM 21介导细胞溶质中的抗体依赖性细胞内中和（ADIN），并提供细胞内免疫应答以保护宿主防御病原体感染。本研究克隆了猪TRIM 21（sTRIM 21）基因，并对其在ADIN中的作用进行了研究。I型干扰素诱导PK-15细胞表达sTRIM 21。sTRIM 21在FMDV特异性抗体存在下限制FMDV感染。此外，sTRIM 21与猪免疫球蛋白G（sFc）的Fc片段融合FMDV的VP 1相互作用，从而引起其降解。RING和SPRY结构域对于sTRIM 21降解sFc融合的VP 1都是必需的。这些结果表明FMDV的细胞内中和特征有助于sTRIM 21的抗病毒活性。sTRIM 21提供了另一种细胞内机制来抑制感染细胞中的FMDV感染。(C)2016爱思唯尔B. V.保留所有权利。
The tripartite motif protein 21 (TRIM21) is a ubiquitously expressed E3 ubiquitin ligase and an intracellular antibody receptor. TRIM21 mediates antibody-dependent intracellular neutralization (ADIN) in cytosol and provides an intracellular immune response to protect host defense against pathogen infection. In this study, swine TRIM21 (sTRIM21) was cloned and its role in ADIN was investigated. The expression of sTRIM21 is induced by type I interferon in PK-15 cells. sTRIM21 restricts FMDV infection in the presence of FMDV specific antibodies. Furthermore, sTRIM21 interacts with Fc fragment of swine immunoglobulin G (sFc) fused VP1 of FMDV and thereby causing its degradation. Both the RING and SPRY domains are essential for sTRIM21 to degrade sFc-fused VP1. These results suggest that the intracellular neutralization features of FMDV contribute to the antiviral activity of sTRIM21. sTRIM21 provide another intracellular mechanism to inhibit FMDV infection in infected cells. (C) 2016 Elsevier B.V. All rights reserved.