Transcriptome profiling reveals response genes for downy mildew resistance in cucumber
Transcriptome profiling reveals response genes for downy mildew resistance in cucumber
复制标题
转录组分析揭示了黄瓜霜霉病抗性的反应基因
DOI：
10.1007/s00425-021-03603-6
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发表时间：
2021-05-01
期刊：
影响因子：
4.3
通讯作者：
Ren, Zhonghai
中科院分区：
文献类型：
--
作者：
Gao, Xinbin;Guo, Pei;Ren, Zhonghai
主要结论我们发现了黄瓜对霜霉病的潜在防御途径。激活活性氧和木质素积累途径的信号传导可能在防御反应中发挥重要作用。通过转录组分析鉴定出多种抗性基因。 摘要 霜霉病(DM)由古巴假霜霉(Pseudoperonosporacubensis)引起，是一种破坏性最强的病害之一，造成黄瓜严重减产。然而，人们对调节糖尿病耐药性的基因和途径仍然知之甚少。在我们的研究中，我们观察到，在P下，高度敏感的自交系53（IL53）比高度抗性的自交系51（IL51）表现出更严重的疾病症状。立方体感染。此外，木质素限制了P的发芽和延伸。立方体和H2O2作为耐药过程中的信号分子均表现出增加，表明激活这些途径的信号可能是IL51和IL53之间耐药性差异的原因。以IL51和IL53为亲本的F2群体中的抗性和易感库的转录组分析表明，一系列差异表达的基因参与防御反应的多种功能：病原体相关分子模式识别、信号转导、活性氧和木质素积累以及转录调节因子。结合生理数据和转录组，我们预测了黄瓜抵抗糖尿病的潜在分子机制。我们的研究为进一步研究黄瓜抗DM的机制奠定了基础。
Main conclusionWe discovered a potential defense pathway of cucumber to downy mildew. The signaling that activates the pathways of ROS and lignin accumulation may play an important role in the defense response. Many resistance genes were identified by transcriptome analysis.AbstractDowny mildew (DM), caused byPseudoperonospora cubensis, is one of the most destructive diseases and causes severe yield losses of cucumber. However, the genes and pathways involved in regulating DM resistance were still poorly understood. In our study, we observed that the highly sensitive inbred line 53 (IL53) exhibited more severe disease symptoms than the highly resistant inbred line 51 (IL51) underP. cubensisinfection. Furthermore, lignin, limiting the germination and extension ofP. cubensis, and H2O2, as a signaling molecule during the resistant process,were both shown to increase, indicating that the signaling that activates these pathways might be responsible for the resistance divergence between IL51 and IL53. Transcriptome analysis, using the resistant and susceptible pools in F2populations with IL51 and IL53 as parents, showed that a series of differentially expressed genes was involved in multiple functions of defense response: pathogen-associated molecular pattern recognition, signal transduction, reactive oxygen species and lignin accumulation, and transcription regulators. Combining physiological data with transcriptomes, we predicted a potential molecular mechanism of cucumber resistance to DM. Our research provided a foundation for further studies on the mechanism of cucumber resistance to DM.