Improved medium for extraction of plasminogen activator from tissue.
Improved medium for extraction of plasminogen activator from tissue.
复制标题
用于从组织中提取纤溶酶原激活剂的改进培养基。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
J. M. Madeja
中科院分区：
文献类型：
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作者：
S. Camiolo;M. Siuta;J. M. Madeja
组织中存在的纤溶酶原激活剂的定量可能在很大程度上取决于用于溶解酶的提取程序。已知硫氰酸钾溶液是一种有效的增溶剂，但其可抑制除纤维蛋白平板以外的测定系统。本文报道了一种同样有效的醋酸盐-去污剂萃取剂，它可用于高灵敏度的偶氮酪蛋白溶解测定程序。结果表明，相对于先前报道的磷酸盐去污剂提取剂，可以从选定的组织中提取激活剂活性的三倍增加。浸提介质含有75 mM醋酸钾、0.3 M NaCl、0.1 M L-精氨酸、10 mM EDTA、0.25% Triton X-100，最终pH值为4.2。
Quantitation of plasminogen activators present in tissue may depend to a large extent on the extraction procedure used to solubilize the enzymes. Potassium thiocyanate solution is known to be an efficient solubilizer, but it can inhibit assay systems other than fibrin plates. An equally effective acetate-detergent extractant is reported here which can be used with the highly sensitive azocaseinolytic assay procedure. The results indicate that a three-fold increase in activator activity can be extracted from selected tissues relative to that previously reported for a phosphate-detergent extractant. The extraction medium contains 75 mM K acetate, 0.3 M NaCl, 0.1 M L-arginine, 10 mM EDTA, 0.25% Triton X-100, final pH 4.2.
纤溶酶原激活剂——纤溶酶系统在角膜溃疡中作用的证据。
影响因子：
4.4
作者：
Berman,M;Leary,R;Gage,J
通讯作者：
Gage,J