Pulmonary oxidative stress response in young children with cystic fibrosis
Pulmonary oxidative stress response in young children with cystic fibrosis
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DOI：
10.1136/thx.52.6.557
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发表时间：
1997-06-01
期刊：
影响因子：
10
通讯作者：
Phelan, P
中科院分区：
文献类型：
--
作者：
Hull, J;Vervaart, P;Phelan, P
研究背景--氧化应激在囊性纤维化的肺损伤中起重要作用.然而，没有直接证据表明囊性纤维化患者的肺氧化应激增加，也没有证据表明炎症对主要的肺抗氧化剂谷胱甘肽的影响。一项研究进行测量这些参数的婴儿和幼儿在存在或不存在肺部inflammation.Methods -32名婴儿和幼儿囊性纤维化的平均（SD）年龄21.4（15.3）个月使用支气管肺泡灌洗（BAL）研究了平均（SD）年龄为21.0（21.2）个月（范围2 -54）的7名非囊性纤维化对照受试者。根据BAL结果，将囊性纤维化组分为有肺炎症（CF-I）和无肺炎症（CF-NI）的组。脂质过氧化氢、总谷胱甘肽和γ-谷氨酰转肽酶水平结果CF-I组BALF中脂质过氧化氢和γ-GT浓度明显高于对照组和CF-NI组，每一个都有相似的参数值（CF-I组与对照组的脂质过氧化氢几何平均值比为5.4（95%置信区间（CI）为1.8 - 15.8），γ-GT为5.2（95% CI 1.4至19.4））。谷胱甘肽的浓度往往是较低的CF-I科目，但差异没有达到统计significant.Conclusions -这些结果表明，囊性纤维化患者的气道暴露于增加氧化应激，这似乎是肺部炎症的后果，而不是部分的主要囊性纤维化缺陷。CF-I组中γ-GT的增加表明细胞外谷胱甘肽可被气道上皮细胞利用的机制。
Background - It has been suggested that oxidative stress contributes to lung injury in cystic fibrosis. There is, however, no direct evidence of increased pulmonary oxidative stress in cystic fibrosis nor of the effects of inflammation on the major pulmonary antioxidant, glutathione. A study was undertaken to measure these parameters in infants and young children in the presence or absence of pulmonary inflammation.Methods - Thirty two infants and young children with cystic fibrosis of mean (SD) age 21.4 (15.3) months (range 2-54) and seven non-cystic fibrosis control subjects of mean (SD) age 21.0 (21.2) months (range 2-54) were studied using bronchoalveolar lavage (BAL). On the basis of the BAL findings the cystic fibrosis group was divided into those with (CF-I) and those without pulmonary inflammation (CF-NI). Levels of lipid hydroperoxide, total glutathione, and gamma-glutamyl transpeptidase (gamma-GT) were then measured in the BAL fluid,Results - The concentrations of Lipid hydroperoxide and gamma-GT in the epithelial Lining fluid were significantly increased in the CF-I group compared with the control and CF-NI groups, each of which had similar values for these parameters (ratio of geometric means for CF-I group versus control for lipid hydroperoxide 5.4 (95% confidence interval (CI) 1.8 to 15.8) and for gamma-GT 5.2 (95% CI 1.4 to 19.4)). The glutathione concentration tended to be lower in the CF-I subjects but the difference did not reach statistical significance.Conclusions - These results demonstrate that the airways in patients with cystic fibrosis are exposed to increased oxidative stress which appears to be a consequence of pulmonary inflammation rather than part of the primary cystic fibrosis defect. The increase in gamma-GT in the CF-I group suggests a mechanism by which extracellular glutathione could be utilised by airway epithelial cells.