RAPID ENZYMATIC ASSAY OF INSOLUBLE AND SOLUBLE DIETARY FIBER
RAPID ENZYMATIC ASSAY OF INSOLUBLE AND SOLUBLE DIETARY FIBER
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DOI：
10.1021/jf00117a003
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发表时间：
1983-01-01
影响因子：
6.1
通讯作者：
SILJESTROM, M
中科院分区：
文献类型：
--
作者：
ASP, NG;JOHANSSON, CG;SILJESTROM, M
本文提出了一种重量-酶法同时测定可溶性和不溶性膳食纤维的方法。这种方法可以在1天内分析10-15个重复样品。主要包括以下步骤：在耐热的α-淀粉酶作用下加热15min，与胃酶在酸性pH下孵化1h，与胰酶在中性pH下孵育1h，用Celite545作为助滤剂过滤掉不溶性膳食纤维。用4倍量的乙醇从滤液中沉淀出可溶性膳食纤维，并以与不溶性膳食纤维相同的方式过滤回收。作为另一种选择，可以在酶孵化后立即进行酒精沉淀。然后，可以在一次过滤中回收可溶和不可溶的成分。所有的膳食纤维成分似乎都已确定。几乎所有的淀粉都被溶解了，但一些蛋白质仍未被消化。微生物酶也进行了测试。当对EEC/IARC合作研究中使用的材料进行分析时，这两个酶系统得出了非常相似的结果。
A gravimetric, enzymatic method for the determination of both soluble and insoluble dietary fiber is presented. This method makes it possible to analyze 10-15 duplicate samples in 1 day. The procedure includes the following main steps: gelantinization by boiling 15 min in the presnce of a heat-stable .alpha.-amylase, incubation with pepsin at acid pH for 1 h and incubation with pancreatin at neutral pH for 1 h. Insoluble dietary fiber is filtered off with Celite 545 as the filter aid. Soluble dietary fiber is precipitated from the filtrate with 4 volumes of ethanol and recovered by filtration in the same way as insoluble dietary fiber. As an alternative the alcohol precipitation can be performed immediately after the enzyme incubations. Both soluble and insoluble components can then be recovered in one single filtration. All dietary fiber components seem to be determined. Practically all starch is solubilized, but some protein remains undigested. Microbial enzymes were also tested. When the materials used in the EEC/IARC collaborative study were analyzed, the 2 enzyme systems gave very similar results.