Clonal rat parathyroid cell line expresses a parathyroid hormone-related peptide but not parathyroid hormone itself.
Clonal rat parathyroid cell line expresses a parathyroid hormone-related peptide but not parathyroid hormone itself.
复制标题
克隆大鼠甲状旁腺细胞系表达甲状旁腺激素相关肽，但不表达甲状旁腺激素本身。
DOI：
10.1016/0006-291x(89)91969-4
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发表时间：
1989
影响因子：
3.1
通讯作者：
Broadus,AE
中科院分区：
文献类型：
--
作者：
Ikeda,K;Weir,EC;Sakaguchi,K;Burtis,WJ;Zimering,M;Mangin,M;Dreyer,BE;Brandi,ML;Aurbach,GD;Broadus,AE
在与恶性肿瘤体液高钙血症综合征相关的肿瘤中发现了一种新型甲状旁腺激素相关肽。随后，编码该肽的 mRNA 被发现在许多正常组织中表达，包括甲状旁腺。使用 Northern 印迹、RNase 保护和免疫化学技术，我们检查了克隆大鼠甲状旁腺细胞系，该细胞系最初是作为研究甲状旁腺细胞生理学的模型系统而开发的。我们发现该细胞系表达甲状旁腺激素相关肽，但不表达甲状旁腺激素本身。甲状旁腺激素相关肽的分泌与细胞外钙浓度成反比，但钙和 1,25-二羟基维生素 D3 似乎都不影响稳态甲状旁腺激素相关肽 mRNA 水平。该克隆系可能被证明是一个有趣的系统，用于研究负责组织特异性甲状旁腺激素和甲状旁腺激素相关肽基因表达的因素。
A novel parathyroid hormone-related peptide has been identified in tumors associated with the syndrome of humoral hypercalcemia of malignancy. Subsequently, mRNAs encoding this peptide have been found to be expressed in a number of normal tissues, including the parathyroids. Using Northern blotting, RNase protection, and immunochemical techniques, we examined a clonal rat parathyroid cell line originally developed as a model system for studying parathyroid cell physiology. We found that this line expresses the parathyroid hormone-related peptide but not parathyroid hormone itself. Secretion of the parathyroid hormone-related peptide varied inversely with extracellular calcium concentration, but neither calcium nor 1,25-dihydroxyvitamin D3appeared to influence steady-state parathyroid hormone-related peptide mRNA levels. This clonal line may prove to be an interesting system for studying the factors responsible for tissue-specific parathyroid hormone and parathyroid hormone-related peptide gene expression.