CHARACTERISTICS OF A MODEL OF MYOCARDIAL-INFARCTION PRODUCED BY CORONARY-ARTERY LIGATION IN THE RAT
CHARACTERISTICS OF A MODEL OF MYOCARDIAL-INFARCTION PRODUCED BY CORONARY-ARTERY LIGATION IN THE RAT
复制标题
DOI：
10.1016/1054-8807(95)00021-v
复制
发表时间：
1995-07-01
影响因子：
3.7
通讯作者：
PFEFFER, MA
中科院分区：
文献类型：
--
作者：
COOPER, CJ;PFEFFER, JM;PFEFFER, MA
冠状动脉结扎大鼠模型被广泛用于研究心肌梗死、心室重构和充血性心力衰竭。在691只永久结扎左冠状动脉后存活的动物中，研究了总梗死面积以及心内膜和心外膜梗死之间的关系。从左心室（LV）的连续组织切片中确定心肌体积，然后进行肌肉和瘢痕周长的平面测量。平均梗死面积为左心室总周长的34.5% ± 13.3%。大鼠存活时间越长，其平均梗死面积越小。心外膜与心内膜梗死面积之比所描述的透壁性为0.78 +/-0.01。随着梗死面积的增加，透壁率增加，其变异性降低。内膜下梗死在小梗死中更常见，内膜梗死面积较小。虽然梗死面积是高度可变的，梗死面积指数内皮梗死面积的变化较小。因此，以内膜梗死面积为指标的脑梗死面积可能代表了一种显著更有效的方法，用于评估治疗对减少梗死面积的影响。
The rat model of coronary artery ligation is widely used to study myocardial infarction, ventricular remodeling, and congestive heart failure. Total infarct size and the relationship between endocardial and epicardial infarction were characterized in 691 animals that survived permanent ligation of the left coronary artery. Infarct size was determined from serial histologic sections of the left ventricle (LV), followed by planimetry of muscle and scar circumferences. Mean infarct size was 34.5% +/- 13.3% of total left ventricular circumference. Rats surviving longer durations to terminal study had a smaller mean infarct size. Infarct transmurality, as described by the ratio of epicardial-to-endocardial infarct sizes, was 0.78 +/- 0.01. The transmurality ratio increased and its variability decreased as infarct size increased. Subendocardial infarcts were more frequent among small infarcts and had smaller areas of endocardial infarction. Although infarct size was highly variable, infarct size indexed to endocardial infarct size was less variable. Infarct size indexed to endocardial infarct size may therefore represent a significantly more powerful method for assessing the effects of treatments to reduce infarct size.