Zfp1, a Cys2His2 zinc finger protein is required for meiosis initiation in Tetrahymena thermophila
Zfp1, a Cys2His2 zinc finger protein is required for meiosis initiation in Tetrahymena thermophila
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Zfp1 是一种 Cys2His2 锌指蛋白，是嗜热四膜虫减数分裂启动所必需的
DOI：
10.1080/15384101.2022.2053449
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发表时间：
2022-03-18
期刊：
影响因子：
4.3
通讯作者：
Miao，Wei
中科院分区：
文献类型：
--
作者：
Zhang，Jing;Tian，Miao;Miao，Wei
摘要减数分裂是真核生物有性生殖过程中一个重要的、高度保守的过程。真核生物减数分裂的启动机制多种多样，转录因子在许多物种中都发挥着重要作用。然而，转录因子在启动纤毛虫减数分裂中的具体功能尚不清楚。在这里，我们表明，一个假定的Cys2His2锌指含转录因子编码的ZFP1基因是特异性表达在有性生殖的四膜虫嗜热。在缺乏ZFP 1的细胞中不启动减数分裂。转录组测序分析表明，Zfp1是许多减数分裂特异性基因表达所必需的。我们的研究结果表明Zfp1可能是T.嗜热菌
ABSTRACT Meiosis is an important and highly conserved process that occurs during eukaryotic sexual reproduction. Diverse mechanisms are responsible for meiosis initiation among eukaryotes, and transcription factors have been established to have an important role in many species. However, the specific function of transcription factors in initiating meiosis in ciliates is unknown. Here we show that a putative Cys2His2 zinc finger-containing transcription factor encoded by the ZFP1 gene is specifically expressed during sexual reproduction in Tetrahymena thermophila. Meiosis is not initiated in the cells lacking ZFP1. Transcriptome sequencing analyses reveal that Zfp1 is required for the expression of many meiosis-specific genes. Our results indicate that Zfp1 could be a transcriptional activator required for meiosis initiation in T. thermophila.