Real-Time Volumetric Phase Monitoring: Advancing Chemical Analysis by Countercurrent Separation.
Real-Time Volumetric Phase Monitoring: Advancing Chemical Analysis by Countercurrent Separation.
复制标题
DOI：
10.1021/acs.analchem.5b01613
复制
发表时间：
2015-07-21
影响因子：
7.4
通讯作者：
Friesen JB
中科院分区：
文献类型：
--
作者：
Pauli GF;Pro SM;Chadwick LR;Burdick T;Pro L;Friedl W;Novak N;Maltby J;Qiu F;Friesen JB
Countercurrent separation (CCS) utilizes the differential partitioning behavior of analytes between two immiscible liquid phases. We introduce the first platform (“CherryOne”) capable of real-time monitoring, metering, and control of the dynamic liquid-liquid CCS process. Automated phase monitoring and volumetrics are made possible with an array of sensors, including the new permittivity-based Phase Metering Apparatus (PMA). Volumetric data for each liquid phase are converted into a dynamic real-time display of stationary phase retention (Sf) and eluent partition coefficients (K), which represent critical parameters of CCS reproducibility. When coupled with the elution-extrusion operational mode (EECCC), automated Sf and K determination empowers untargeted and targeted applications ranging from metabolomic analysis to preparative purifications.
