EFFICIENT TRANSFORMATION OF RICE (ORYZA-SATIVA L) MEDIATED BY AGROBACTERIUM AND SEQUENCE-ANALYSIS OF THE BOUNDARIES OF THE T-DNA
EFFICIENT TRANSFORMATION OF RICE (ORYZA-SATIVA L) MEDIATED BY AGROBACTERIUM AND SEQUENCE-ANALYSIS OF THE BOUNDARIES OF THE T-DNA
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DOI：
10.1046/j.1365-313x.1994.6020271.x
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发表时间：
1994-08-01
期刊：
影响因子：
7.2
通讯作者：
KUMASHIRO, T
中科院分区：
文献类型：
--
作者：
HIEI, Y;OHTA, S;KUMASHIRO, T
通过农杆菌介导的水稻组织共培养，获得了大量形态正常、可育的转基因水稻植株。转化效率与常规用于用细菌转化双子叶植物的方法所获得的转化效率相似。通过对R（0）、R（1）和R（2）代转化子的分子和遗传分析，证明了转基因的稳定整合、表达和遗传。序列分析表明，转基因水稻植株的T-DNA边界与转基因双子叶植物的T-DNA边界基本一致。由小盾叶诱导的愈伤组织是非常好的起始材料。A.菌株。携带所谓的“超二元”载体的根癌农杆菌对包括越光（Koshihikari）在内的各种粳稻品种的转化频率特别高，越光通常在组织培养中表现出较差的反应。
A large number of morphologically normal, fertile, transgenic rice plants were obtained by co-cultivation of rice tissues with Agrobacterium tumefaciens. The efficiency of transformation was similar to that obtained by the methods used routinely for transformation of dicotyledons with the bacterium. Stable integration, expression and inheritance of transgenes were demonstrated by molecular and genetic analysis of transformants in the R(0), R(1) and R(2) generations. Sequence analysis revealed that the boundaries of the T-DNA in transgenic rice plants were essentially identical to those in transgenic dicotyledons. Calli induced from scutella were very good starting materials. A strain of A. tumefaciens that carried a so-called 'super-binary' vector gave especially high frequencies of transformation of various cultivars of japonica rice that included Koshihikari, which normally shows poor responses in tissue culture.