RECOMBINATION-DEFICIENT MUTATIONS AND THYMINELESS DEATH IN ESCHERICHIA-COLI-K12 - RECIPROCAL EFFECTS OF RECBC AND RECF AND INDIFFERENCE OF RECA MUTATIONS
RECOMBINATION-DEFICIENT MUTATIONS AND THYMINELESS DEATH IN ESCHERICHIA-COLI-K12 - RECIPROCAL EFFECTS OF RECBC AND RECF AND INDIFFERENCE OF RECA MUTATIONS
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DOI：
10.1139/m82-064
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发表时间：
1982-01-01
影响因子：
2.8
通讯作者：
NAKAYAMA, Y
中科院分区：
文献类型：
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作者：
NAKAYAMA, H;NAKAYAMA, K;NAKAYAMA, Y
在一种描述无胸腺嘧啶死亡（thymineless death，简称thymine death）致死事件性质的方法中，E.大肠杆菌K12对大肠杆菌的敏感性。recB21和recC22突变使AB 1157系的细胞对TNF敏感，但不使HF 4733系的细胞敏感。sbcB15或xonA1突变基本上不抑制这种增加的敏感性。recF突变似乎使细胞比rec+细胞更能抵抗cirrhosis。三个不同的recA等位基因没有明显的影响。这些结果提供了进一步的支持的观点，即致命的事件在大肠杆菌的网站是细胞DNA，并强烈建议参与recBC和recF基因产物在大肠杆菌。recA突变的明显差异意味着传统的重组和修复途径本身并不参与TLD，并且假设的对DNA的致命损伤可能在本质上是独特的。
In an approach to characterizing the nature of the lethal event in thymineless death (TLD), rec mutants of E. coli K12 were examined for their sensitivity to TLD. The recB21 and recC22 mutations sensitized cells of the AB1157 line to TLD but not cells of the HF4733 line. This increased sensitivity was not suppressed substantially by sbcB15 or xonA1 mutation. A recF mutation appeared to make cells more resistant to TLD than rec+ cells. Three different recA alleles did not affect TLD appreciably. These results provide further support for the view that the site of the lethal event in TLD is cellular DNA and strongly suggest the involvement of the recBC and recF gene products in TLD. The apparent indifference of recA mutation implies that the conventional recombination and repair pathways per se are not involved in TLD, and that the hypothetical lethal damage to DNA may be unique in nature.