Vitamin B2 (riboflavin) increases drought tolerance of Agaricus bisporus
Vitamin B2 (riboflavin) increases drought tolerance of Agaricus bisporus
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DOI：
10.1080/00275514.2017.1414544
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发表时间：
2017-01-01
期刊：
影响因子：
2.8
通讯作者：
Weig, Alfons R.
中科院分区：
文献类型：
--
作者：
Guhr, Alexander;Horn, Marcus A.;Weig, Alfons R.
干旱是许多土壤生物的压力源。虽然植物对干旱适应机制进行了广泛的研究，但真菌的信息很少。抗氧化剂是特别相关的，因为干燥伴随着细胞内活性氧的过度产生。核黄素（维生素B-2）是一种调节植物耐旱性的抗氧化剂。类似的功能可能存在于真菌中。在这里，我们研究了双孢蘑菇的呼吸和转录响应干旱和核黄素的影响。建立了四组围隔实验：菌丝在干旱或无干旱条件下用或不用50 μ M核黄素处理。干旱增加核黄素含量在菌丝约5倍，但也没有，除了核黄素。在没有添加核黄素的情况下，在约-20 MPa的水势下，真菌呼吸降低了50%以上。添加核黄素后，呼吸作用仍高出约2-3倍。仅干旱或仅核黄素的转录反应强烈重叠，主要基于转录和翻译调控因子。这在联合治疗中更强。核黄素诱导干旱胁迫菌丝的保护机制。最明显的是甲基乙二醛（糖酵解的细胞毒性副产物）的乳酰谷胱甘肽裂解酶解毒。因此，我们的数据表明，胁迫启动功能和核黄素在干旱反应的A。双孢菌。
Drought is a stressor for many soil-inhabiting organisms. Although plants have been extensively investigated for drought-adaptive mechanisms, little information is available for fungi. Antioxidants are especially relevant, since desiccation is accompanied by an excessive intracellular production of reactive oxygen species. Riboflavin (vitamin B-2) is one antioxidant regulating drought tolerance in plants. A similar function may exist in fungi. Here, we examined the respiratory and transcriptional responses of Agaricus bisporus to drought and the impact of riboflavin. Mesocosm experiments with four groups were established: hyphae were treated with or without 50 mu M riboflavin under drought or no drought conditions. Drought increased riboflavin content in hyphae about 5 times with, but also without, addition of riboflavin. Without addition of riboflavin, fungal respiration decreased by more than 50% at water potentials of about -20 MPa. With addition of riboflavin, respiration remained about 2-3 times higher. The transcriptional responses to only drought or only riboflavin strongly overlapped and were mainly based on factors regulating transcription and translation. This was even stronger in combined treatments. Riboflavin induced protective mechanisms in drought-stressed hyphae. Most pronounced was the methylglyoxal (cytotoxic by-product of glycolysis) detoxifying of lactoylglutathione lyase. Thus, our data suggest a stress-priming function and a role of riboflavin in drought responses of A. bisporus.