The Arabidopsis thaliana PARTING DANCERS gene encoding a novel protein is required for normal meiotic homologous recombination.
The Arabidopsis thaliana PARTING DANCERS gene encoding a novel protein is required for normal meiotic homologous recombination.
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DOI：
10.1091/mbc.e05-09-0902
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发表时间：
2005-12
影响因子：
3.3
通讯作者：
A. Wijeratne;Changbin Chen;Wei Zhang;L. Timofejeva;Hong Ma
中科院分区：
文献类型：
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作者：
A. Wijeratne;Changbin Chen;Wei Zhang;L. Timofejeva;Hong Ma
最近对出芽酵母和模式植物拟南芥减数分裂重组的研究表明，减数分裂交叉（COs）通过两条遗传途径发生：干扰敏感途径和干扰不敏感途径。然而，很少有基因在这两种途径中被发现。在这里，我们描述了离别舞者（PTD）基因的鉴定，作为一个基因在减数细胞中表达水平升高。对PTD中两个独立产生的转移DNA插入系的分析表明，突变体的育性降低。进一步的细胞学分析显示，ptd突变体的雄性减数分裂存在缺陷，包括交叉形成减少，COs的细胞学外观。突变体中残留的交叉是随机分布的，表明ptd突变体在干扰敏感途径中CO的形成存在缺陷。此外，对突变体的透射电镜分析未发现突触复合物的明显异常，在粗线期未发现明显正常的晚期重组结节，这表明突变体的二价形成缺陷是突触后的。与其他序列相似性有限的基因相比，PTD可能在不同的真核生物中呈现出一种以前未知的保守功能。
Recent studies of meiotic recombination in the budding yeast and the model plant Arabidopsis thaliana indicate that meiotic crossovers (COs) occur through two genetic pathways: the interference-sensitive pathway and the interference-insensitive pathway. However, few genes have been identified in either pathway. Here, we describe the identification of the PARTING DANCERS (PTD) gene, as a gene with an elevated expression level in meiocytes. Analysis of two independently generated transferred DNA insertional lines in PTD showed that the mutants had reduced fertility. Further cytological analysis of male meiosis in the ptd mutants revealed defects in meiosis, including reduced formation of chiasmata, the cytological appearance of COs. The residual chiasmata in the mutants were distributed randomly, indicating that the ptd mutants are defective for CO formation in the interference-sensitive pathway. In addition, transmission electron microscopic analysis of the mutants detected no obvious abnormality of synaptonemal complexes and apparently normal late recombination nodules at the pachytene stage, suggesting that the mutant's defects in bivalent formation were postsynaptic. Comparison to other genes with limited sequence similarity raises the possibility that PTD may present a previously unknown function conserved in divergent eukaryotic organisms.