Effect of surface roughness on the hydrophobicity of a denture-base acrylic resin and Candida albicans colonization
Effect of surface roughness on the hydrophobicity of a denture-base acrylic resin and Candida albicans colonization
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DOI：
10.1111/jicd.12125
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发表时间：
2016-05-01
影响因子：
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通讯作者：
Vergani, Carlos E.
中科院分区：
文献类型：
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作者：
de Foggi, Camila C.;Machado, Ana L.;Vergani, Carlos E.
目的：本研究的目的是评估表面粗糙度（平均粗糙度[Ra] μ m）对义齿基托丙烯酸树脂疏水性以及白色念珠菌（C.方法：将标本分为Ra 0.05、Ra 0.2、Ra 0.4、Ra 0.8、Ra 1.5、Ra 3.0六组。测定了水接触角（WCA），并与C.白色念珠菌90 min（初始粘附，n = 108）或48 h（生物膜形成，n = 108）。通过c.f.u./ mL和2，3-二-（2-甲氧基-4-硝基-5-磺基苯基）-2H-四氮唑-5-甲酰苯胺（XTT），并对两种方法进行相关性评价。结果：Ra 0.05和3.0组WCA均值分别为最低（与75度相似）和最高（与100度相似）。对于初始粘附和生物膜形成，如通过c.f.u./ mL和XTT。SEM分析表明，粗糙度显著增加了C.白色念珠菌
Aim: The aim of the present study was to evaluate the effect of surface roughness (roughness average [Ra] mu m) on the hydrophobicity of a denture-base acrylic resin and the initial adherence and biofilm formation of Candida albicans (C. albicans).Methods: Disk-shaped specimens were divided into six groups: Ra 0.05, Ra 0.2, Ra 0.4, Ra 0.8, Ra 1.5, and Ra 3.0. Water contact angles (WCA) were measured, and the specimens incubated with C. albicans for 90 min (initial adherence, n = 108) or 48 h (biofilm formation, n = 108). Adhered and biofilm cells were evaluated by c.f.u./mL and 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), and the correlation between the two methods was evaluated. The surface of the specimens and cells (adhered and biofilm) were also analyzed by scanning electron microscopy (SEM).Results: Groups Ra 0.05 and 3.0 exhibited the lowest (similar to 75 degrees) and the highest (similar to 100 degrees) WCA mean values, respectively. For both initial adherence and biofilm formation, no statistically-significant differences were observed among all groups, as determined by c.f.u./mL and XTT. A positive correlation between these two methods was found. SEM analysis showed the presence of scratches and valleys on the acrylic specimens and densely-packed yeast cells covering the entire surface.Conclusions: Roughness significantly increased hydrophobicity (WCA), but had no effect on the number and metabolic activity of adherent and biofilm cells of C. albicans.