Identification of the origin of natural alcohols by natural abundance hydrogen-2 nuclear magnetic resonance
Identification of the origin of natural alcohols by natural abundance hydrogen-2 nuclear magnetic resonance
复制标题
自然丰度氢2核磁共振鉴定天然醇的来源
DOI：
10.1021/ac00250a057
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发表时间：
1982
影响因子：
7.4
通讯作者：
M. Michon
中科院分区：
文献类型：
--
作者：
G. Martin;Maryvonne L. Martin;F. Mabon;M. Michon
在水/二氧六环峰出现后，木糖（k ′ = 1.2）与核糖（k ′ = 2.9）和果糖（k ′ = 3.5）基线分离，这两种物质可明显分离。使用硼酸盐络合物形成来产生阴离子糖物质导致选择性的显著改善。葡萄糖-硼酸盐复合物的形成常数（~ 102）远小于相应的果糖复合物（~ 104）（7）。因此，在本文所用的实验条件下，葡萄糖不会产生任何可检测的响应，使得果糖的测定完全不受葡萄糖干扰。改变这些条件以适应葡萄糖复合物的较低稳定性，例如通过增加硼酸根离子浓度，也很可能允许测定葡萄糖。尽管需要进一步的工作来明确建立分离所有糖所需的最佳条件，但似乎在柱上发生反应以得到用于有效分离和检测的合适产物。本实验室正在沿着这些路线进行进一步的工作。
Following the appearance of the water/dioxane peaks, there is base line separation of xylose (k'= 1.2) from ribose (k'= 2.9) and fructose (k'= 3.5) which are clearly separable. The use of borate complexformation to produce anionic sugar species results in a dramatic improvement in selectivity. The formation constant for the glucose-borate complex is far smaller (—102) than the corresponding fructose complex (~ 104)(7). As a result, glucose does not give any detectable response under the experimental conditions employed here, making the determination of fructose entirely free from glucose interference. Changing these conditions to accommodate the lower stability of the glucose complex, eg, by increasing borate ion concentration, would very likely permit determination of glucose also. Although further work is required to clearly establish the op-timum conditions necessary to separate all sugars, it would appear that reactions occur on the column to give suitable products for effective separation and detection. Further work along these lines is under way in this laboratory.