Digital, Three-dimensional Average Shaped Atlas of the Heliothis Virescens Brain with Integrated Gustatory and Olfactory Neurons.
Digital, Three-dimensional Average Shaped Atlas of the Heliothis Virescens Brain with Integrated Gustatory and Olfactory Neurons.
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DOI：
10.3389/neuro.06.014.2009
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发表时间：
2009
影响因子：
3
通讯作者：
Mustaparta H
中科院分区：
文献类型：
--
作者：
Kvello P;Løfaldli BB;Rybak J;Menzel R;Mustaparta H
我们以菜蛾为模型生物，研究了神经网络在化学感觉编码和学习中的作用。组成神经元的特征是细胞内记录结合染色，导致在每个大脑准备中识别出一个神经元。为了在空间上将不同制剂的神经元联系起来，需要一个共同的大脑框架。我们在这里展示了一份平均形状的蛾脑图谱。它基于11个女性大脑标本，每个标本都染色了一个荧光突触标记，并在共聚焦激光扫描显微镜下进行扫描。在计算机软件Amira中手动重建每个制备的脑神经毛发，然后使用迭代形状平均程序生成图谱。为了展示图谱的应用，我们记录了两个嗅觉和两个味觉中间神经元，以及味觉感受器神经元到图谱的轴突投射，可视化了它们的空间关系。嗅觉中间神经元呈典型的内束触角叶投射神经元形态，投射于蘑菇体的花盏内，外侧投射于原脑叶内。这两个味觉中间神经元分别对蔗糖和奎宁作出反应，投射到大脑的不同区域。奎宁反应神经元的广泛投射包括与嗅觉中间神经元投射相邻的外侧区域。蔗糖反应神经元局限于食道下神经节，树枝状树枝状分支与味觉感受器神经元在鼻部的轴突投射重叠。通过作为神经元整合的工具，该图谱提供了三维空间中神经元之间的空间关系的视觉途径，从而促进了对绿色日光脑神经元网络的研究。飞蛾的标准大脑可以在
We use the moth Heliothis virescens as model organism for studying the neural network involved in chemosensory coding and learning. The constituent neurons are characterised by intracellular recordings combined with staining, resulting in a single neuron identified in each brain preparation. In order to spatially relate the neurons of different preparations a common brain framework was required. We here present an average shaped atlas of the moth brain. It is based on 11 female brain preparations, each stained with a fluorescent synaptic marker and scanned in confocal laser-scanning microscope. Brain neuropils of each preparation were manually reconstructed in the computer software Amira, followed by generating the atlas using the Iterative Shape Average Procedure. To demonstrate the application of the atlas we have registered two olfactory and two gustatory interneurons, as well as the axonal projections of gustatory receptor neurons into the atlas, visualising their spatial relationships. The olfactory interneurons, showing the typical morphology of inner-tract antennal lobe projection neurons, projected in the calyces of the mushroom body and laterally in the protocerebral lobe. The two gustatory interneurons, responding to sucrose and quinine respectively, projected in different areas of the brain. The wide projections of the quinine responding neuron included a lateral area adjacent to the projections of the olfactory interneurons. The sucrose responding neuron was confined to the suboesophageal ganglion with dendritic arborisations overlapping the axonal projections of the gustatory receptor neurons on the proboscis. By serving as a tool for the integration of neurons, the atlas offers visual access to the spatial relationship between the neurons in three dimensions, and thus facilitates the study of neuronal networks in the Heliothis virescens brain. The moth standard brain is accessible at