Syntaxin 6 functions in trans-Golgi network vesicle trafficking
Syntaxin 6 functions in trans-Golgi network vesicle trafficking
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DOI：
10.1091/mbc.8.7.1261
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发表时间：
1997-07-01
影响因子：
3.3
通讯作者：
Scheller, RH
中科院分区：
文献类型：
--
作者：
Davanger, S;Bock, JB;Scheller, RH
囊泡在细胞器之间的特异性转移对于产生和维持细胞内膜隔室的组织至关重要。Syntaxin 6是最近发现的syntaxin家族成员，其成分是几种囊泡运输途径的必需组分。为了更好地理解syntaxin 6的功能，我们产生了一组单克隆抗体，它们特异性地识别该蛋白质的不同表位。免疫电子显微镜显示syntaxin 6主要在trans-Golgi网络（TGN）上，在那里它与网格蛋白包被的膜上的TGN衔接蛋白AP-1部分共定位。额外的标记存在于内体样结构附近的小泡上。免疫沉淀的syntaxin 6显示，它是存在于一个复杂的或复合物与α-可溶性NSF附着蛋白，囊泡相关膜蛋白2，或cellubrevin和哺乳动物的同源物VPS 45，这是一个成员的sec 1家族牵连在高尔基体的prevacuolar室运输酵母。我们发现，哺乳动物VPS 45被发现在多个组织中，是部分膜相关的，并在高尔基体区域富集。越来越多的证据表明，突触融合蛋白6介导了TGN运输事件，可能靶向哺乳动物细胞中的内体。
The specific transfer of vesicles between organelles is critical in generating and maintaining the organization of membrane compartments within cells. Syntaxin 6 is a recently discovered member of the syntaxin family, whose constituents are required components of several vesicle trafficking pathways. To better understand the function of syntaxin 6, we generated a panel of monoclonal antibodies that specifically recognize different epitopes of the protein. Immunoelectron microscopy shows syntaxin 6 primarily on the trans-Golgi network (TGN), where it partially colocalizes with the TGN adapter protein AP-1 on clathrin-coated membranes. Additional label is present on small vesicles in the vicinity of endosome-like structures. Immunoprecipitation of syntaxin 6 revealed that it is present in a complex or complexes with alpha-soluble NSF attachment protein, vesicle-associated membrane protein 2, or cellubrevin and a mammalian homologue of VPS45, which is a member of the sec1 family implicated in Golgi to prevacuolar compartment trafficking in yeast. We show that mammalian VPS45 is found in multiple tissues, is partially membrane associated, and is enriched in the Golgi region. Converging lines of evidence suggest that syntaxin 6 mediates a TGN trafficking event, perhaps targeting to endosomes in mammalian cells.