Comparison and utilization of different PCR-based approaches for molecular typing of Pseudomonas syringae pv. actinidiae strains from Italy
Comparison and utilization of different PCR-based approaches for molecular typing of Pseudomonas syringae pv. actinidiae strains from Italy
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DOI：
10.1080/07060661.2010.538556
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发表时间：
2011-01-01
影响因子：
2
通讯作者：
Balestra, Giorgio M.
中科院分区：
文献类型：
--
作者：
Mazzaglia, Angelo;Renzi, Marsilio;Balestra, Giorgio M.
在过去的几年里，意大利报道了一种影响猕猴桃的严重细菌性疾病。其病原经鉴定为假单胞菌pv.锕系元素。出于流行病学的目的，我们评估了各种基于PCR的指纹识别方法的区分能力。通过rep-PCR（（GTG）5-PCR）、IS 50-PCR和RAPD从意大利、韩国和日本的广泛收集菌株中生成图谱。不同致病力菌株间的遗传关系。对猕猴桃属和相关致病变种的菌株进行了调查。意大利菌株之间未发现差异。然而，意大利菌株与日本和韩国菌株之间存在一些差异。其他致病型分别聚在一起，pv.茶与PV的关系总是更密切。比其他人更强烈。在一般情况下，用不同的方法得到的结果是在良好的协议，与RAPD组之间的分歧显示最高值。
Over the last few years, a severe bacterial disease affecting kiwifruit has been reported in Italy. Its causal agent was identified as Pseudomonas syringae pv. actinidiae. For epidemiological purposes, we evaluated the discriminatory power of various PCR-based fingerprinting approaches. Profiles were generated by rep-PCR ((GTG)5-PCR), IS50-PCR and RAPD, from a wide collection of Italian, Korean and Japanese strains. The genetic relationships between strains of pv. actinidiae and strains from related pathovars were investigated. No differences between Italian strains were identified. However, some differences were identified between Italian strains and strains from Japan and Korea. The other pathovars clustered separately, and pv. theae was always more closely related to pv. actinidiae than the others. In general, the results obtained with the different methods were in good agreement, with RAPD showing the highest values of divergence between groups.