Ly-6C is expressed in brain vessels endothelial cells but not in microglia of the mouse
Ly-6C is expressed in brain vessels endothelial cells but not in microglia of the mouse
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DOI：
10.1016/s0304-3940(98)00473-x
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发表时间：
1998-07-17
影响因子：
2.5
通讯作者：
Pessac, B
中科院分区：
文献类型：
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作者：
Alliot, F;Rutin, J;Pessac, B
Ly-6C抗原在免疫系统的各种细胞类型中表达，包括巨噬细胞。使用特异性识别Ly-6C的单克隆抗体ER-MP20，我们研究了脑实质小胶质细胞是否在体内以及体外表达Ly-6C，在来自发育和成年C57/B1小鼠的脑切片中，所有血管均被强烈免疫标记。电镜免疫组化显示内皮细胞为表达Ly-6C的细胞类型。相反，我们从未观察到免疫反应性的小胶质细胞，但是，小胶质细胞增殖在体外强烈ER-MP20阳性。这些数据表明，Ly-6C不是体内小胶质细胞的标志物。(C)1998爱思唯尔科学爱尔兰有限公司保留所有权利。
The Ly-6C antigen is expressed in various cell types of the immune system, including macrophages. Using the monoclonal antibody ER-MP20 which specifically recognises Ly-6C, we have investigated whether brain parenchymatous microglia express Ly-6C in vivo as well as in vitro, in brain sections from developing and adult C57/Bl mice, all vessels were strongly immunolabelled. Electron microscopic immunohistochemistry showed that the endothelial cells are the cell type expressing Ly-6C. In contrast, we never observed immunoreactivity on microglia; however, microglial cells proliferating in vitro were strongly ER-MP20 positive. These data show that Ly-6C is not a marker for microglia in vivo. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.