ROS‑induced PCD affects the viability of seeds with different moisture content after cryopreservation
ROS‑induced PCD affects the viability of seeds with different moisture content after cryopreservation
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ROS诱导的PCD对不同含水量种子超低温保存后活力的影响
DOI：
10.1007/s11240-021-02219-8
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Yan Liu
中科院分区：
文献类型：
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作者：
Ruifen Ren;Hao Zhou;Lingling Zhang;Xueru Jiang;Min Zhang;Yan Liu
水分含量是影响种子超低温保存的重要因素之一，但水分含量如何影响种子超低温保存后的活力尚不完全清楚。本研究比较了不同含水量野生芍药种子超低温保存前后程序性细胞死亡（PCD）和活性氧（ROS）的变化及其与活力的关系，探讨ROS诱导的PCD对不同含水量超低温保存种子活力的作用。结果表明：超低温保存后，随着含水量的增加，种子活力显着下降，ROS含量增加。出现细胞质壁分离、细胞膜破裂、核皱缩等PCD典型形态特征； caspase-3样蛋白酶活性显着升高，线粒体膜电位降低，参与PCD的发生。种子冷冻保存后经外源过氧化氢（H2O2）处理，显着促进PCD的发生，显着降低种子活力；然而，外源ROS清除剂抗坏血酸（AsA）的孵育却产生相反的效果。这些结果表明，ROS 诱导的 PCD 影响了冷冻保存后的种子活力。不同含水量的种子超低温保存后ROS浓度不同，PCD发生程度不同，导致超低温保存后活力存在差异。
Moisture content is one of the important factors affected the cryopreservation of seeds, but it is not completely clear how moisture content affects the viability of seeds after cryopreservation. In this study, compared the changes of programmed cell death (PCD) and reactive oxygen species (ROS) of wildPaeonia emodiseeds with different moisture content before and after cryopreservation, and their relationship with viability, to investigate the role of ROS- induced PCD in seed viability after cryopreservation with different moisture content. The results showed that: seed viability significantly decreased and the ROS content increased with the increase of moisture content after cryopreservation. With the appearance of typical morphological characteristics of PCD such as cytoplasmic wall separation, cell membrane rupture and nuclear shrinkage; the activity of caspase-3-like protease was significantly increased and mitochondrial membrane potential decreased, which involved in the occurrence of PCD. The seeds were treated with exogenous hydrogen peroxide (H2O2) after cryopreserved, which significantly promoted the occurrence of PCD and significantly reduced the viability of seeds; however, the incubation of exogenous ROS scavenger ascorbic acid (AsA) had the opposite effects. These results indicated that ROS induced PCD affected the seed viability after cryopreservation. The ROS concentration of seeds with different moisture content was different after cryopreservation, and the occurrence degree of PCD was different, which leaded to the difference of viability after cryopreservation.