Apolipoprotein M associates to lipoproteins through its retained signal peptide
Apolipoprotein M associates to lipoproteins through its retained signal peptide
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DOI：
10.1016/j.febslet.2008.02.007
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发表时间：
2008-03-05
期刊：
影响因子：
3.5
通讯作者：
Dahlback, Bjorn
中科院分区：
文献类型：
--
作者：
Axler, Olof;Ahnstrom, Josefin;Dahlback, Bjorn
载脂蛋白M（apoM）主要与HDL相关。在这项研究中，研究了apoM的未切割信号肽是否是该蛋白质与脂蛋白结合的能力所必需的。在HEK293细胞中表达具有可裂解信号肽Q22A的ApoM以及野生型apoM。在分子排阻色谱上，野生型apoM的洗脱曲线与人HDL相关血浆apoM的洗脱曲线相似。相比之下，Q22A突变体的大小对应于游离的、未缔合的apoM。这有力地表明，信号肽确实是apoM与脂质结合的能力所必需的。(c)2008年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
Apolipoprotein M (apoM) is predominantly associated with HDL. In this study, it was investigated whether apoM's uncleaved signal peptide is necessary for the protein's ability to associate with lipoproteins. ApoM with a cleavable signal peptide, Q22A, was expressed, together with wild-type apoM, in HEK293 cells. On size-exclusion chromatography, the elution profile of wild-type apoM was similar to that of human HDL-associated plasma apoM. In contrast, the size of the Q22A mutant corresponded to free, unassociated apoM. This strongly indicates that the signal peptide is indeed necessary for apoM's ability to associate with lipid. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.