REORGANIZATION OF ARRAYS OF PREKERATIN FILAMENTS DURING MITOSIS - IMMUNOFLUORESCENCE MICROSCOPY WITH MULTICLONAL AND MONOCLONAL PREKERATIN ANTIBODIES
REORGANIZATION OF ARRAYS OF PREKERATIN FILAMENTS DURING MITOSIS - IMMUNOFLUORESCENCE MICROSCOPY WITH MULTICLONAL AND MONOCLONAL PREKERATIN ANTIBODIES
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DOI：
10.1016/0014-4827(81)90427-4
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发表时间：
1981-01-01
影响因子：
3.7
通讯作者：
GEIGER, B
中科院分区：
文献类型：
--
作者：
HORWITZ, B;KUPFER, H;GEIGER, B
间接免疫荧光标记不同上皮细胞系的预角蛋白型中间丝显示有丝分裂过程中预角蛋白组织的显著变化。在4个细胞系（人胚胎肠细胞Henle-407、人上皮癌A-431和人宫颈癌HeLa细胞）中，有3个细胞系在有丝分裂开始时丝状预角蛋白网络消失，免疫荧光标记集中在小细胞质体中。兔抗牛前角蛋白多特异性抗体和小鼠杂交瘤产生的单特异性抗体均获得了这一观察结果。在第4个细胞系[大鼠袋鼠肾PtK2细胞]PtK2中，预角蛋白丝在整个有丝分裂过程中被保留，主要在有丝分裂的两极，而细胞的中心区域明显没有丝。在不同的培养细胞中添加秋水仙碱可引起角蛋白前丝组织的改变，通常表现为形成较粗的丝束。它不能诱导间期细胞形成预角化蛋白-细胞质体。在秋水仙碱的存在下长时间孵育后，有丝分裂阻滞的细胞数量增加，含有角蛋白前细胞质体的细胞数量也相应增加。显然，这些细胞中预角蛋白的组织状态依赖于细胞周期，并可能被调节以允许有丝分裂期间发生的根本形状变化。
Indirect immunofluorescent labeling of different epithelial cell lines for intermediate filaments of the prekeratin type revealed prominent changes in the organization of prekeratin during mitosis. In 3 out of 4 cell lines tested [human embryonic intestinal cell] Henle-407, [human epitheloid carcinoma] A-431 and [human cervical carcinoma cell] HeLa cells) the filamentous prekeratin networks disappeared at the initiation of mitosis and the immunofluorescent labeling was concentrated in small cytoplasmic bodies. This observation was obtained with both polyspecific rabbit anti-bovine prekeratin antibodies and with monospecific antibodies produced by mouse hybridomas. In a 4th cell line, [rat kangaroo kidney PtK2 cell] PtK2, prekeratin filaments were retained throughout mitosis, mainly in the mitotic poles, whereas the central areas of the cells were apparently devoid of filaments. The addition of colchicine to the different cultured cells induced alterations in the organization of prekeratin filaments which were usually manifested by the formation of thicker filament bundles. It did not induce the formation of the prekeratin-cytoplasmic bodies in interphase cells. Upon prolonged incubation in the presence of colchicine, there was an increase in the number of mitotically arrested cells and a parallel increase in the number of cells containing prekeratin cytoplasmic bodies. Apparently, the state of organization of prekeratin in these cells is cell-cycle-dependent and may be modulated to permit radical shape changes as those occurring during mitosis.