A novel RNA-binding protein in neuronal RNA granules: Regulatory machinery for local translation
A novel RNA-binding protein in neuronal RNA granules: Regulatory machinery for local translation
复制标题
DOI：
10.1523/jneurosci.0382-05.2005
复制
发表时间：
2005-04-27
影响因子：
5.3
通讯作者：
Tokunaga, M
中科院分区：
文献类型：
--
作者：
Shiina, N;Shinkura, K;Tokunaga, M
神经元树突内的局部翻译是突触长时程可塑性的重要基础，树突内的RNA颗粒参与了局部翻译。在这里，我们确定RNG105（RNA颗粒蛋白105），一种新的RNA结合蛋白，作为海马神经元树突中的RNA颗粒的组成部分。RNG105定位RNA颗粒含有mRNA，其翻译产物在突触可塑性中起关键作用。RNG 105具有在体外和体内抑制翻译的能力，这与RNA颗粒在基础条件下被抑制的发现一致。RNG105从RNA颗粒的解离由BDNF诱导，BDNF是一种负责突触可塑性的生长因子。RNG105的解离与颗粒附近的局部翻译的诱导一致。这些研究结果表明，RNG105是一个翻译抑制剂的RNA颗粒，并提供洞察RNG105动力学和本地翻译调节之间的联系。
Local translation in neuronal dendrites is an important basis for long-term synaptic plasticity, and RNA granules in the dendrites are involved in the local translation. Here, we identify RNG105 ( RNA granule protein 105), a novel RNA-binding protein, as a component of the RNA granules in dendrites of hippocampal neurons. The RNG105-localizing RNA granules contain mRNAs, the translational products of which play key roles in synaptic plasticity. RNG105 has an ability to repress translation both in vitro and in vivo, consistent with the finding that the RNA granule is translationally arrested in the basal conditions. Dissociation of RNG105 from the RNA granules is induced by BDNF, a growth factor responsible for synaptic plasticity. The RNG105 dissociation is coincident with the induction of local translation near the granules. These findings suggest that RNG105 is a translational repressor in the RNA granules and provide insight into the link between RNG105 dynamics and local translational regulation.