Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions.
Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions.
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DOI：
10.1007/978-1-4939-6393-5_17
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发表时间：
2016
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影响因子：
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通讯作者：
Palecek, Sean P
中科院分区：
文献类型：
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作者：
Bao, Xiaoping;Lian, Xiaojun;Palecek, Sean P
Efficient derivation of endothelial cells and their progenitors from human pluripotent stem cells (hPSCs) can facilitate further studies of human vascular development, disease modeling, drug discovery and cell-based therapy. Here we provide a detailed protocol for directing hPSCs to functional endothelial cells and their progenitors in a completely defined, growth factor- and serum-free system by temporal modulation of Wnt/β-catenin signaling via small molecules. We demonstrate a 10-day, two-stage process that recapitulates endothelial cell development, in which hPSCs first differentiate to endothelial progenitors that then generate functional endothelial cells and smooth muscle cells. Methods to characterize endothelial cell identity and function are also described.
