Characterization of low dose streptozotocin-induced progressive diabetes in mice
Characterization of low dose streptozotocin-induced progressive diabetes in mice
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DOI：
10.1016/s1382-6689(00)00064-8
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发表时间：
2001-01-01
影响因子：
4.3
通讯作者：
Naruse, A
中科院分区：
文献类型：
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作者：
Ito, M;Kondo, Y;Naruse, A
我们之前的研究表明，一个单一的IP。链脲佐菌素(STZ)100 mg/kg可诱导成年ICR小鼠慢性进展性糖尿病。在本研究中，注射100 mg/kg STZ的小鼠在注射STZ后24周的整个观察过程中，非空腹血清胰岛素水平均正常。在注射STZ的小鼠中，胰岛面积和胰岛素免疫反应细胞(β细胞)的数量在第1周时正常，然后随着时间的推移继续减少。相比之下，这些小鼠的胰升糖素免疫反应细胞(阿尔法细胞)的数量相对增加。此外，在服用STZ的小鼠中，糖耐量从2周持续下降到实验结束时的12周。在给予STZ的小鼠中，葡萄糖刺激的血清胰岛素水平从大约2周开始下降，并在12周时完全停止。这些结果表明，100 mg/kg STZ诱导的糖尿病小鼠模型为非胰岛素依赖型糖尿病，其特征是胰岛素对葡萄糖刺激的反应减弱。(C)2001 Elsevier Science B.V.保留所有权利。
Our previous study indicated that a single i.p. injection of 100 mg/kg streptozotocin (STZ) is able to induce slowly progressive diabetes mellitus in adult ICR mice. In the present study, the non-fasting serum insulin levels of the mice administered 100 mg/kg STZ were normal throughout the 24-week-observation after STZ injection. In the STZ-administered mice, the area of islets and the number of insulin-immunoreactive cells (beta -cells) were normal at I week and then continued to decrease gradually as the time went on. In contrast, there was a relative increase in the number of glucagon-immunoreactive cells (alpha -cells) in these mice. In addition, in the STZ-administered mice, the degree of glucose tolerance continued to reduce from 2 weeks till 12 weeks when the experiment terminated. The rise in serum insulin levels stimulated by glucose in the STZ-administered mice began to subside from about 2 weeks and had completely ceased by 12 weeks. These results indicate that 100 mg/kg STZ-induced diabetic mouse model is non-insulin-dependent diabetes, which is characterized by impaired insulin response to glucose stimulation. (C) 2001 Elsevier Science B.V. All rights reserved.