TRANSPARENT TESTA8 Inhibits Seed Fatty Acid Accumulation by Targeting Several Seed Development Regulators in Arabidopsis
TRANSPARENT TESTA8 Inhibits Seed Fatty Acid Accumulation by Targeting Several Seed Development Regulators in Arabidopsis
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TRANSPARENT TESTA8 通过针对拟南芥中的几种种子发育调节剂来抑制种子脂肪酸积累。
DOI：
10.1104/pp.114.235507
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发表时间：
2014-06-01
期刊：
影响因子：
7.4
通讯作者：
Jiang, Lixi
中科院分区：
文献类型：
--
作者：
Chen, Mingxun;Xuan, Lijie;Jiang, Lixi
脂肪酸（FAs）和脂肪酸衍生的复合脂在植物生长和营养发育中起着重要作用，是储存在成熟种子中的一类重要代谢物。控制植物种子中FA积累的因素和调控网络在很大程度上仍然未知。透明TESTA8 （TT8）在类黄酮生物合成和种皮颜色形成中的调控作用被广泛研究；然而，其影响种子FA生物合成的功能尚不清楚。在本文中，我们发现拟南芥（Arabidopsis thaliana） TT8通过下调一组对胚胎发育至关重要或在FA生物合成途径中重要的基因来影响种子FA的生物合成并抑制种子FA的积累。此外，tt8突变导致种子成熟过程中蛋白质沉积减少。TT8-糖皮质激素受体融合蛋白的翻译后激活和染色质免疫沉淀实验表明，TT8抑制了对种子发育至关重要的关键转录因子叶型子叶don1、叶型子叶don2和FUSCA3的活性，以及介导甘油脂生物合成的胞苷二磷酸二酰基甘油合成酶e2的活性。这些结果有助于我们了解TT8的整个功能，并增加我们对植物种子中fa衍生的复杂脂质形成的复杂网络的了解。
Fatty acids (FAs) and FA-derived complex lipids play important roles in plant growth and vegetative development and are a class of prominent metabolites stored in mature seeds. The factors and regulatory networks that control FA accumulation in plant seeds remain largely unknown. The role of TRANSPARENT TESTA8 (TT8) in the regulation of flavonoid biosynthesis and the formation of seed coat color is extensively studied; however, its function in affecting seed FA biosynthesis is poorly understood. In this article, we show that Arabidopsis (Arabidopsis thaliana) TT8 acts maternally to affect seed FA biosynthesis and inhibits seed FA accumulation by down-regulating a group of genes either critical to embryonic development or important in the FA biosynthesis pathway. Moreover, the tt8 mutation resulted in reduced deposition of protein in seeds during maturation. Posttranslational activation of a TT8-GLUCOCORTICOID RECEPTOR fusion protein and chromatin immunoprecipitation assays demonstrated that TT8 represses the activities of LEAFY COTYLEDON1, LEAFY COTYLEDON2, and FUSCA3, the critical transcriptional factors important for seed development, as well as CYTIDINEDIPHOSPHATE DIACYLGLYCEROL SYNTHASE2, which mediates glycerolipid biosynthesis. These results help us to understand the entire function of TT8 and increase our knowledge of the complicated networks regulating the formation of FA-derived complex lipids in plant seeds.