The effect of synthetic alginate sulfate hydrogels with recombinant PDGF-BB on Wound healing
The effect of synthetic alginate sulfate hydrogels with recombinant PDGF-BB on Wound healing
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DOI：
10.4149/bll_2018_072
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发表时间：
2018-01-01
影响因子：
1.5
通讯作者：
Shakeri, F.
中科院分区：
文献类型：
--
作者：
Babavalian, H.;Tebyanian, H.;Shakeri, F.
目的：血小板源性生长因子（PDGF-BB）是促进创面再生和愈合的重要因子。问题之一是在伤口部位中这些因子的传递或递送没有足够的效率。本研究以重组PDGF-BB生长因子为载体合成海藻酸钠-硫酸盐水凝胶，以实现创伤快速愈合的方法。MTT法检测其毒性。研究了转化生长因子从合成的海藻酸钠硫酸盐水凝胶中的释放，并且确认了无毒性，并且用重组人生长因子制备了水凝胶。在大鼠背部造成直径为10 mm的伤口，以检查伤口愈合情况。结果：本研究表明，藻酸盐硫酸盐水凝胶-PDGF-BB比非硫酸盐藻酸盐水凝胶-PDGF-BB在伤口愈合方面更快。结论：含重组生长因子4 μ g/cm（2）的藻酸盐硫酸盐水凝胶（PDGF-BB）非常适合伤口愈合（表1，图3，参考文献34）。PDF文本www.elis.sk。
OBJECTIVE: Platelet-derived growth factor (PDGF-BB) is an important factor in the regeneration and wound healing. One of the problems is that there is not enough effi ciency in the transmission or delivery of such factors in the wound site. In this study, alginate sulfate hydrogel was synthesized with recombinant PDGF-BB growth factor for achieving a quick method in wound healing.METHODS: In this study, Alginate sulfate hydrogel was made by photo-crosslinking method and methacrylate. Its toxicity was evaluated by MTT assay. Transforming growth factor was studied in releasing from synthesized alginate sulfate hydrogels and also, lack of toxicity was confi rmed, and hydrogel was made with a recombinant human growth factor. Wounds were created with a diameter of 10 mm on the back of rats in order to check the wound healing.RESULTS: This study showed that alginate sulfate hydrogels-PDGF-BB were faster in wound healing than non-sulfate alginate hydrogels-PDGF-BB. Therefore, the controlled delivery of growth factor system can be a powerful idea for therapeutic applications for wound healing.CONCLUSION: Alginate sulfate hydrogel with recombinant growth factor 4 mu g/cm(2) (PDGF-BB) was very suitable for wound healing (Tab.1, Fig.3, Ref.34). Text in PDF www.elis.sk.