Somatic embryogenesis from cultured epicotyls and primary leaves of soybean [Glycine max (L.) Merrill]
Somatic embryogenesis from cultured epicotyls and primary leaves of soybean [Glycine max (L.) Merrill]
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培养的大豆上胚轴和初生叶的体细胞胚胎发生 [Glycine max (L.) Merrill]
DOI：
10.1007/s11627-997-0002-1
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
J. Pellow
中科院分区：
文献类型：
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作者：
K. Rajasekaran;J. Pellow
已经证明了几种大豆品种[Glycine max(L.) Merrill]通过培养的上胚轴和初生叶的体细胞胚胎发生进行再生。当具有完整合子胚轴的子叶半部在补充有 10 mg 1−1(45.2 µM) 2,4-D 的 Murashige 和 Skoog (MS) 培养基上培养时，从上胚轴和初生叶诱导体细胞胚胎发生。在补充有 20 mg 1−1(90.5 µM) 2,4-二氯苯氧基乙酸 (2,4-D) 的 MS 培养基上，由小群体细胞胚胎建立稳定、持续增殖的球状胚胎培养物 (GEC)。在含有 2.5 mg 1−1(11.3 µM) 6-苄基腺嘌呤的 Cheng 基础培养基 (CBO) 上，实现了发芽体细胞胚胎芽尖的快速增殖。可育植物从个体体细胞胚和体外繁殖的不定芽培养物中获得。
Regeneration of several varieties of soybean [Glycine max(L.) Merrill] by somatic embryogenesis from cultured epicotyls and primary leaves has been demonstrated. Somatic embryogenesis was induced from epicotyls and primary leaves when cotyledon halves with the intact zygotic embryo axes were cultured on Murashige and Skoog (MS) medium supplemented with 10 mg 1−1(45.2 µM) 2,4-D. Stable, continuously proliferating globular embryo cultures (GEC) were established from small groups of somatic embryos on MS medium supplemented with 20 mg 1−1(90.5 µM) 2,4-dichlorophenoxyacetic acid (2,4-D). Rapid multiplication of shoot tips from germinating somatic embryos was achieved on Cheng’s basal medium (CBO) containing 2.5 mg 1−1(11.3 µM) 6-benzyladenine. Fertile plants were obtained from individual somatic embryos andin vitropropagated adventitious shoot bud cultures.