Gene expression analysis reveals that Delta/Notch signalling is not involved in onychophoran segmentation.
Gene expression analysis reveals that Delta/Notch signalling is not involved in onychophoran segmentation.
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DOI：
10.1007/s00427-016-0529-4
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发表时间：
2016-03
影响因子：
2.4
通讯作者：
Budd GE
中科院分区：
文献类型：
--
作者：
Janssen R;Budd GE
Delta/Notch （Dl/N）信号参与了脊椎动物（可能也包括环节动物和节肢动物）切分过程的基因调控网络，导致了切分可能在两侧动物的最后共同祖先中进化的假设。然而，由于在研究充分的节肢动物中看似矛盾的结果，Dl/N信号在分割中的作用和起源通常仍然不清楚。在这项研究中，我们通过基因表达分析研究了与节肢动物有亲缘关系的onychophoran Euperipatoides kanangrensis的Dl/N信号传导的核心成分。我们发现，无论是Delta或Notch，还是其信号通路的任何其他研究成分，都不可能参与掌掌类动物的片段添加。相反，我们认为Dl/N信号可能参与后伸长，这是这些基因的另一个保守功能。我们进一步提出，可能是后伸长网络，而不是经典的Dl/N信号，控制着爪龙高度保守的片段极性基因网络和较低水平的配对规则基因网络。因此，我们认为，配对规则基因网络及其与Dl/N信号的相互作用可能是在节肢动物谱系中进化而来的，因此Dl/N信号可能是在不同门的片段添加中独立募集的。本文的在线版本（doi:10.1007/s00427-016-0529-4）包含补充材料，仅供授权用户使用。
Delta/Notch (Dl/N) signalling is involved in the gene regulatory network underlying the segmentation process in vertebrates and possibly also in annelids and arthropods, leading to the hypothesis that segmentation may have evolved in the last common ancestor of bilaterian animals. Because of seemingly contradicting results within the well-studied arthropods, however, the role and origin of Dl/N signalling in segmentation generally is still unclear. In this study, we investigate core components of Dl/N signalling by means of gene expression analysis in the onychophoran Euperipatoides kanangrensis, a close relative to the arthropods. We find that neither Delta or Notch nor any other investigated components of its signalling pathway are likely to be involved in segment addition in onychophorans. We instead suggest that Dl/N signalling may be involved in posterior elongation, another conserved function of these genes. We suggest further that the posterior elongation network, rather than classic Dl/N signalling, may be in the control of the highly conserved segment polarity gene network and the lower-level pair-rule gene network in onychophorans. Consequently, we believe that the pair-rule gene network and its interaction with Dl/N signalling may have evolved within the arthropod lineage and that Dl/N signalling has thus likely been recruited independently for segment addition in different phyla. The online version of this article (doi:10.1007/s00427-016-0529-4) contains supplementary material, which is available to authorized users.