Effect of epidermal growth factor on the synthetic activity of human fibroblasts.
Effect of epidermal growth factor on the synthetic activity of human fibroblasts.
复制标题
表皮生长因子对人成纤维细胞合成活性的影响。
DOI：
10.1016/0304-4165(80)90080-x
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Page,RC
中科院分区：
文献类型：
--
作者：
Huey,J;Narayanan,AS;Jones,K;Page,RC
研究了表皮生长因子对人牙龈成纤维细胞DNA和蛋白质合成的影响。与10%胎牛血清相比，表皮生长因子处理的同步细胞合成的DNA量显著增加，合成高峰出现的时间比血清晚6小时。表皮生长因子引起蛋白质合成的剂量依赖性刺激（脯氨酸掺入）。胶原合成不受影响，因此，胶原合成的比例随着表皮生长因子浓度的增加而降低。阿司匹林和吲哚美辛没有消除这些作用，表明前列腺素可能没有参与。
The influence of epidermal growth factor on DNA and protein synthesis by human gingival fibroblasts was studied. Synchronized cells treated with epidermal growth factor synthesized considerably greater amounts of DNA relative to 10% fetal calf serum and the peak of synthesis occurred 6 h later than with serum. Epidermal growth factor caused a dose-dependent stimulation of protein synthesis (proline incorporation). Collagen synthesis remained unaffected and, as a result, the proportion of collagen synthesized decreased with increasing epidermal growth factor concentration. Aspirin and indomethacin did not abrogate these effects, indicating that prostaglandins may not be involved.