Acute exposure to diesel exhaust induces central nervous system stress and altered learning and memory in honey bees.
Acute exposure to diesel exhaust induces central nervous system stress and altered learning and memory in honey bees.
复制标题
急性暴露于柴油机尾气会引起中枢神经系统应激，并改变蜜蜂的学习和记忆能力。
DOI：
10.1038/s41598-019-41876-w
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发表时间：
2019
影响因子：
4.6
通讯作者：
Reitmayer CM
中科院分区：
文献类型：
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作者：
Reitmayer CM
为了有效地觅食，许多昆虫传粉者依赖于学习和回忆花香的能力，这些行为与一系列复杂的细胞过程有关。在这里，我们研究了如何急性暴露于高剂量的柴油机废气（含有19.8和17.5 ppm的NO和NO2，分别）影响蜜蜂（意大利蜜蜂）的联想学习行为和表达的一种普遍存在的热休克蛋白，HSP 70，在他们的中枢神经系统（CNS）。为了确定暴露于柴油机废气是否会改变它们对随后的非生物胁迫的耐受性，我们进一步使个体经受热应激。柴油废气暴露降低蜜蜂学习和回忆条件气味刺激的能力。虽然在整个实验期间（3.5 h）暴露于柴油机废气或清洁空气的蜜蜂之间CNS HSP70表达没有显著差异，但时间和暴露处理与时间之间存在显著的相互作用。这种相互作用进行了研究，使用相关性分析，这表明，只有在柴油机废气暴露的蜜蜂有一个显着的正相关性HSP 70的表达和时间。此外，与清洁空气对照个体相比，能够回忆起柴油暴露后72小时气味的蜜蜂个体减少了44%。此外，柴油机尾气对A.意利费拉因一种方式，降低了他们的生存能力，第二个后续的压力。空气污染对学习、记忆和应激耐受力的负面影响有可能降低个体蜜蜂的觅食效率和授粉成功率。
For effective foraging, many insect pollinators rely on the ability to learn and recall floral odours, behaviours that are associated with a complex suite of cellular processes. Here, we investigated how acute exposure to a high-dose of diesel exhaust (containing 19.8 and 17.5 ppm of NO and NO2, respectively) affected associative learning behaviour of honey bees (Apis mellifera) and expression of a ubiquitous heat shock protein, HSP70, in their central nervous system (CNS). To determine whether exposure to diesel exhaust would alter their tolerance to a subsequent abiotic stress, we further subjected individuals to heat stress. Diesel exhaust exposure decreased honey bees’ ability to learn and recall a conditioned odour stimulus. Whilst there was no significant difference in CNS HSP70 expression between honey bees exposed to either diesel exhaust or clean air across the entire duration of the experiment (3.5 h), there was a significant effect of time and a significant interaction between exposure treatment and time. This interaction was investigated using correlation analyses, which demonstrated that only in the diesel exhaust exposed honey bees was there a significant positive correlation between HSP70 expression and time. Furthermore, there was a 44% reduction in honey bee individuals that were able to recall the odour 72 h after diesel exposure compared with clean air control individuals. Moreover, diesel exhaust affectedA. melliferain a way that reduced their ability to survive a second subsequent stressor. Such negative effects of air pollution on learning, recall, and stress tolerance has potential to reduce foraging efficiency and pollination success of individual honey bees.