Fission yeast IQGAP arranges actin filaments into the cytokinetic contractile ring
Fission yeast IQGAP arranges actin filaments into the cytokinetic contractile ring
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DOI：
10.1038/emboj.2009.252
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发表时间：
2009-10-21
期刊：
影响因子：
11.4
通讯作者：
Nakano, Kentaro
中科院分区：
文献类型：
--
作者：
Takaine, Masak;Numata, Osamu;Nakano, Kentaro
收缩环（CR）由成束的肌动蛋白丝和肌球蛋白II组成;然而，肌动蛋白成束因子仍然难以捉摸。我们表明，裂殖酵母裂殖酵母IQGAP Rng 2参与产生的CRF-肌动蛋白，并需要其安排成一个环。Rng 2的N-末端片段对于Rng 2的功能是必需的，并且定位于CRF-肌动蛋白。在体外，该片段促进肌动蛋白聚合并形成F-肌动蛋白线性阵列，这些阵列对肌动蛋白解聚因子Adf 1诱导的解聚具有抵抗力。我们的研究结果表明，Rng 2参与产生的CR F-肌动蛋白，同时捆绑的细丝，并通过抵消Adf 1的影响，从而使重建的CR F-肌动蛋白束，这提供了一个洞察到的物理性质的积木，包括CR调节其动态。The EMBO Journal（2009）28，3117-3131. doi：10.1038/doj.2009.252; 2009年8月27日在线发布
The contractile ring (CR) consists of bundled actin filaments and myosin II; however, the actin-bundling factor remains elusive. We show that the fission yeast Schizosaccharomyces pombe IQGAP Rng2 is involved in the generation of CR F-actin and required for its arrangement into a ring. An N-terminal fragment of Rng2 is necessary for the function of Rng2 and is localized to CR F-actin. In vitro the fragment promotes actin polymerization and forms linear arrays of F-actin, which are resistant to the depolymerization induced by the actin-depolymerizing factor Adf1. Our findings indicate that Rng2 is involved in the generation of CR F-actin and simultaneously bundles the filaments and regulates its dynamics by counteracting the effects of Adf1, thus enabling the reconstruction of CR F-actin bundles, which provides an insight into the physical properties of the building blocks that comprise the CR. The EMBO Journal (2009) 28, 3117-3131. doi: 10.1038/emboj.2009.252; Published online 27 August 2009