Amperometric glucose biosensor based on electrodeposition of platinum nanoparticles onto covalently immobilized carbon nanotube electrode
Amperometric glucose biosensor based on electrodeposition of platinum nanoparticles onto covalently immobilized carbon nanotube electrode
复制标题
基于铂纳米粒子电沉积到共价固定碳纳米管电极上的电流葡萄糖生物传感器。
DOI：
10.1016/j.talanta.2006.09.013
复制
发表时间：
2007-03-30
期刊：
影响因子：
6.1
通讯作者：
Yu, Ruqin
中科院分区：
文献类型：
--
作者：
Chu, Xia;Duan, Daxue;Yu, Ruqin
基于葡萄糖氧化酶（GOx）在金、铂纳米粒子修饰碳纳米管（CNT）电极上的吸附，研制了一种新型葡萄糖电流型生物传感器。碳纳米管通过碳二亚胺化学反应与半胱胺自组装膜（SAM）的氨基残基形成酰胺键，共价固定在金电极上。制备的GOx/Au-nano/Pt-nano/CNT电极上覆盖一层薄薄的Nafion，以避免测定中GOx的损失并提高抗干扰能力。采用石英晶体微天平技术对碳纳米管在金电极上的固定化进行了表征。用扫描电子显微镜（SEM）研究了CNT/Au和Pt-nano/CNT/Au电极的形貌，用电流法研究了Au、CNT/Au、Pt-nano/Au和Pt-nano/CNT/Au电极的电化学性能。此外，还讨论了Pt纳米粒子电沉积时间、pH值、外加电位和电活性干扰物对传感器响应的影响，在无介体存在下，该酶电极对葡萄糖具有良好的电催化活性和快速响应。线性范围为0.5至17.5 mM，校正系数为0.996。该传感器对葡萄糖的测定具有良好的重现性和稳定性。(c)2006 Elsevier B. V.保留所有权利。
A new amperometric biosensor for glucose was developed based on adsorption of glucose oxidase (GOx) at the gold and platinum nanoparticles-modified carbon nanotube (CNT) electrode. CNTs were covalently immobilized on gold electrode via carbodiimide chemistry by forming amide linkages between carboxylic acid groups on the CNTs and amine residues of cysteamine self-assembled monolayer (SAM). The fabricated GOx/Au-nano/Pt-nano/CNT electrode was covered with a thin layer of Nafion to avoid the loss of GOx in determination and to improve the anti-interferent ability. The immobilization of CNTs on the gold electrode was characterized by quartz crystal microbalance technique. The morphologies of the CNT/gold and Pt-nano/CNT/gold electrodes have been investigated by scanning electron microscopy (SEM), and the electrochemical performance of the gold, CNT/gold, Pt-nano/gold and Pt-nano/CNT/gold electrodes has also been studied by amperometric method. In addition, effects of electrodeposition time of Pt nanoparticles, pH value, applied potential and electroactive interferents on the amperometric response of the sensor were discussed.The enzyme electrode exhibited excellent electrocatalytic activity and rapid response for glucose in the absence of a mediator. The linear range was from 0.5 to 17.5 mM with correction coefficient of 0.996. The biosensor had good reproducibility and stability for the determination of glucose. (c) 2006 Elsevier B.V. All rights reserved.