Contributions to automated trace analysis. Part II. Rapid method for the automated determination of lead in whole blood by electrothermal atomic-absorption spectrophotometry.
Contributions to automated trace analysis. Part II. Rapid method for the automated determination of lead in whole blood by electrothermal atomic-absorption spectrophotometry.
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对自动跟踪分析的贡献。
DOI：
10.1039/an9780300714
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
T. Rains
中科院分区：
文献类型：
--
作者：
M. Stoeppler;K. Brandt;T. Rains
本文介绍了一种简便、快速的原子吸收分光光度法测定全血中铅的方法。用2 M硝酸处理等份（25-200 µl）手指针刺和静脉样本，以进行脱蛋白和基质修饰。离心后，取上清液进行铅的自动分析。用独立方法检查方法的准确度，结果令人满意。因此，确定可获得的准确度约为30%，几乎在精密度的置信区间（p= 0.05）内。对282对随机选择的常规样品进行的评价表明其精密度可接受：例如，正常水平100 µg l-1的相对标准偏差为8.4%，升高水平480 µg l-1的相对标准偏差为3.47%。缩短的体温程序每天可进行多达370次测量，计算机连接可在职业和筛查方案期间立即进行数据评估，并可进行24小时测量。
A simple, rapid method is described for the determination of lead in whole blood by means of electrothermal atomic-absorption spectrophotometry. Aliquots (25–200 µl) of fingerprick and venous samples were treated with 2 M nitric acid for deproteinisation and matrix modification. After centrifuging, the supernatant was taken for the automated analysis for lead. The accuracy of the method was checked with independent methods and found to be satisfactory. Thus it was established that the accuracy obtainable of ⩽30% lies almost within the confidence intervals (p= 0.05) of precision. An evaluation of 282 pairs of randomly selected routine samples indicated an acceptable precision: the relative standard deviation for, for example, the normal level of 100 µg l–1 is 8.4%, and for an elevated level of 480 µg l–1 is 3.47%. The shortened temperature programme makes up to 370 measurements per day possible and the computer coupling permits an immediate data evaluation during occupational and screening programmes and makes possible round-the-clock measurements.