Fecal calprotectin as a measure of disease activity in childhood inflammatory bowel disease
Fecal calprotectin as a measure of disease activity in childhood inflammatory bowel disease
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DOI：
10.1097/00005176-200102000-00015
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发表时间：
2001-02-01
影响因子：
2.9
通讯作者：
Golden, BE
中科院分区：
文献类型：
--
作者：
Bunn, SK;Bisset, WM;Golden, BE
背景：钙保护素是一种丰富的中性粒细胞蛋白，在粪便中非常稳定。本研究的目的是通过与改良的劳埃德-斯蒂尔和格林评分和实验室炎症指标的比较，评价粪便钙保护素作为儿童炎症性肠病(IBD)疾病活动性的无创性指标的有效性。方法：对37例IBD儿童和31名对照儿童的粪便样本进行现场采集。用普通邮件寄到实验室。粪便钙保护素浓度测定采用室内酶联免疫吸附试验(EL ISA)。结果：16例溃疡性结肠炎患儿和21例克罗恩病患儿的粪便钙保护素水平分别为11.5,272.5 mg/L和14.0，0.7-59.7 mg/L，与对照组的中位数(2.1，0.5-6.3 mg/L，P<；0.001)相比，差异有统计学意义(P<；0.001)。12名“中度感染”儿童(35-65岁)的粪便钙保护素浓度(22.22.7-141.7 mg/L)高于25名“轻度感染”儿童(65岁)(10.30.6-272.5 mg/L，P=0.002)。对于全面性IBD组，粪便钙保护素浓度与MLSS呈负相关(r=-0.61，P<；0.001)。与血清白蛋白浓度呈负相关(r=-0.002.49，P=0.0 1)，与血沉呈正相关(r=0.40，P=0.0 1)。作为一种简单、安全、非侵入性的测试，它有可能减少对这些儿童进行侵入性检查的次数。
Background: Calprotectin is an abundant neutrophil protein that is extremely stable in feces. The aim of this study was to assess the effectiveness of fecal calprotectin as a noninvasive measure of disease activity in childhood inflammatory bowel disease (IBD) by comparison to a modified Lloyd-Still and Green score and laboratory inflammatory indices.Methods: Spot fecal samples from 37 children with IBD and 31 control children were sent. by ordinary mail to the laboratory. Fecal calprotectin concentration was measured by an inhouse enzyme linked immunosorbent assay (ELISA). A modified Lloyd-Still & Green score (mLSS) was calculated for each child with IBD within 10 days of obtaining the fecal sample.Results: Compared with control values (median, range) (2.1, 0.5-6.3 mg/L), fecal calprotectin was increased in 16 children with ulcerative colitis, (11.5, 0.6-272.5 mg/L, P < 0.001) and in 21 children with Crohn disease, (14.0, 0.7-59.7 mg/L, P < 0.001). Twelve "moderately affected" children (mLSS of 35-65) had higher fecal calprotectin concentrations (22.2, 2.7-141.7 mg/L) than 25 "mildly affected" children (mLSS > 65), (10.3, 0.6-272.5 mg/L, P = 0.002). For the total IBD group, fecal calprotectin concentration correlated negatively with the mLSS (r = - 0.61, P < 0.001). It also correlated negatively with serum albumin concentration (r = - 0.49, P = 0.002) and positively with erythrocyte sedimentation rate (r = 0.40, P = 0.01).Conclusions: Fecal calprotectin seems to reflect bowel inflammation in children with IBD. As a simple, safe, noninvasive test, it has the potential to reduce the number of invasive investigations performed in these children.