Transcriptome Profile Based on Protein-Protein Interaction Networks Provides a Set of Core Genes for Understanding the Metabolic Mechanisms of the Egg-Protecting Behavior in Amphioctopus fangsiao
Transcriptome Profile Based on Protein-Protein Interaction Networks Provides a Set of Core Genes for Understanding the Metabolic Mechanisms of the Egg-Protecting Behavior in Amphioctopus fangsiao
复制标题
基于蛋白-蛋白相互作用网络的转录组图谱为了解芳小两栖章鱼保卵行为的代谢机制提供了一组核心基因
DOI：
10.1007/s11802-022-4999-x
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发表时间：
2022-10-01
影响因子：
1.6
通讯作者：
Li Zan，Zan
中科院分区：
文献类型：
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作者：
Bao Xiaokai，Xiaokai;Li Zan，Zan
Marine organisms cannot grow and reproduce without proper metabolic regulation. Within a metabolic network, problems with a given link will affect the normal life activities of the organism. Many metabolic mechanisms associated with behaviors ofAmphioctopus fangsiaoare still unclear. Moreover, as a factor affecting the normal growth ofA. fangsiao, egg protection has rarely been considered in previous behavioral studies. In this research, we analyzed the transcriptome profile of gene expression inA. fangsiaoegg-unprotected larvae and egg-protected larvae, and identified 818 differentially expressed genes (DEGs). We used GO and KEGG enrichment analyses to search for metabolism-related DEGs. Protein-protein interaction networks were constructed to examine the interactions between metabolism-related genes. Twenty hub genes with multiple protein-protein interaction relationships or that were involved in multiple KEGG signaling pathways were obtained and verified by quantitative RT-PCR. We first studied the effects of egg protection on the metabolism ofA. fangsiaolarvae by means of protein-protein interaction networks, and the results provide valuable gene resources for understanding the metabolism of invertebrate larvae. The data serve as a foundation for further research on the egg-protecting behavior of invertebrates.
