The Rice TAL Effector-Dependent Resistance Protein XA10 Triggers Cell Death and Calcium Depletion in the Endoplasmic Reticulum
The Rice TAL Effector-Dependent Resistance Protein XA10 Triggers Cell Death and Calcium Depletion in the Endoplasmic Reticulum
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DOI：
10.1105/tpc.113.119255
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发表时间：
2014-01-01
期刊：
影响因子：
11.6
通讯作者：
Yin, Zhongchao
中科院分区：
文献类型：
--
作者：
Tian, Dongsheng;Wang, Junxia;Yin, Zhongchao
植物抗病基因(R)和病原菌同源无毒基因(AVR)之间的识别可以产生局部细胞程序性死亡的超敏反应。然而，我们对植物中R基因介导的过敏性反应的早期信号事件的了解仍然有限。在这里，我们报道了水稻白叶枯病抗性相关基因Xa10的克隆和鉴定。Xa10在其启动子中含有TAL效应子AvrXa10(EBEAvrXa10)的结合元件，AvrXa10特异性地诱导Xa10的表达。Xa10基因的表达诱导水稻、本氏烟草和哺乳动物HeLa细胞的程序性死亡。Xa10基因产物Xa10作为六聚体定位于内质网，与植物和HeLa细胞中内质网钙离子的耗竭有关。在N.benthamiana和HeLa细胞中取消程序性细胞死亡和内质网钙离子耗竭的XA10变异体也取消了水稻的抗病能力。我们认为XA10是一种可诱导的内在终结者蛋白，通过一种保守的机制触发细胞程序性死亡，该机制涉及破坏内质网和细胞内钙稳态。
The recognition between disease resistance (R) genes in plants and their cognate avirulence (Avr) genes in pathogens can produce a hypersensitive response of localized programmed cell death. However, our knowledge of the early signaling events of the R gene-mediated hypersensitive response in plants remains limited. Here, we report the cloning and characterization of Xa10, a transcription activator-like (TAL) effector-dependent R gene for resistance to bacterial blight in rice (Oryza sativa). Xa10 contains a binding element for the TAL effector AvrXa10 (EBEAvrXa10) in its promoter, and AvrXa10 specifically induces Xa10 expression. Expression of Xa10 induces programmed cell death in rice, Nicotiana benthamiana, and mammalian HeLa cells. The Xa10 gene product XA10 localizes as hexamers in the endoplasmic reticulum (ER) and is associated with ER Ca2+ depletion in plant and HeLa cells. XA10 variants that abolish programmed cell death and ER Ca2+ depletion in N. benthamiana and HeLa cells also abolish disease resistance in rice. We propose that XA10 is an inducible, intrinsic terminator protein that triggers programmed cell death by a conserved mechanism involving disruption of the ER and cellular Ca2+ homeostasis.