Utilisation of intramyocellular lipids (IMCLs) during exercise as assessed by proton magnetic resonance spectroscopy (1H-MRS)
Utilisation of intramyocellular lipids (IMCLs) during exercise as assessed by proton magnetic resonance spectroscopy (1H-MRS)
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DOI：
10.1055/s-2001-12407
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发表时间：
2001-02-01
影响因子：
2.2
通讯作者：
Jacob, S
中科院分区：
文献类型：
--
作者：
Brechtel, K;Niess, AM;Jacob, S
最近，H-1-MRS方法可用于非侵入性地定量肌细胞内脂质（IMCL）。目前，对这种脂质池的调节知之甚少。在中等强度的长时间运动期间，非血浆来源的脂肪酸作为能量来源发挥重要作用;位于骨骼肌内的脂质被认为是这些脂肪酸的主要来源。为了了解运动是否会降低IMCL，对12名男性跑步者在不同负荷和持续时间的运动前后进行了研究。6名受试者参加了非竞争性跑步（NCR），3名跑步者参加了竞争性半程马拉松（HM，21 km），另外3名参加了竞争性马拉松（M，42 km）。通过H-1-MR波谱在运动回合之前和之后在胫骨前肌（TA）和比目鱼肌（SOL）中量化肌细胞内和肌细胞外脂质。中等强度（MI; NCR中60- 70%VO2max），平均运动时间（MET）范围在105-110分钟之间，使两块肌肉的IMCL降低10 - 36%。长时间的MI运动（MET 210-240 min; 68 - 70%VO2max，M）使TA的IMCL降低42 - 57%，SOL降低27-56%。相反，高强度运动（HI; MET 80 - 120 min; 83 - 85%VO2max，HM）未改变任何肌肉的IMCL。肌细胞外脂质（EMCL）在任何组中均未显示任何显著变化。数据显示，一轮中等强度（50- 70%VO2max）的有氧运动显着降低ICL在TA和SOL肌肉的时间依赖性的方式评估H-1-MRS。然而，类似的持续时间，但更高的工作量（> 80%VO2max）的运动并没有减少IMCL。这些数据表明，运动持续时间和工作量都是决定IMCL减少的重要因素。
Recently, a H-1-MRS method became available to quantify intramyocellular lipids (IMCL) non-invasively. Currently, little is known about the regulation of this lipid pool. During prolonged exercise of moderate intensity, non-plasma-derived fatty acids play an important role as an energy source; lipids located within the skeletal muscle are considered to be a major source for these fatty acids. To see whether IMCL are reduced by exercise, 12 male runners were studied before and after exercising at different workloads and duration. Six subjects participated in a non-competitive run (NCR), three runners in a competitive half marathon (HM, 21 km) and another three in a competitive marathon (M, 42 km). Intra- and extramyocellular lipids were quantified by H-1-MR spectroscopy in the tibialis anterior (TA) and soleus (SOL) muscles prior to and after the exercise bout. Moderate intensity (MI; 60-70% VO2max in NCR) with a mean exercise time (MET) ranging between 105-110 min decreased IMCL by 10 - 36% in both muscles. Prolonged MI exercise (MET 210-240 min; 68 - 70 % VO2max in M) reduced IMCL by 42 - 57 % in TA and 27-56% in SOL, In contrast, high intensity exercise (HI; MET 80 - 120 min; 83 - 85 % VO2max in HM) did not alter IMCL in either muscle. Extramyocellular lipids (EMCL) did not show any significant change in any group. The data show that one bout of moderate-intensity (50-70% VO2max) aerobic exercise markedly reduces the ICL in TA and SOL muscles in a time-dependent fashion as assessed by H-1-MRS. However, exercise of similar duration but higher workload (> 80% VO2max) does not reduce IMCL. These data suggest that both exercise duration and workload are important factors in determining the reduction of IMCL.