Epidermal response of the Japanese eel to environmental stress
Epidermal response of the Japanese eel to environmental stress
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DOI：
10.1023/a:1007746514851
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发表时间：
1998-10-01
影响因子：
2.9
通讯作者：
Hirose, H
中科院分区：
文献类型：
--
作者：
Aranishi, F;Mano, N;Hirose, H
通过测定鳗鱼表皮提取物的各种裂解活性，监测日本鳗鱼对环境胁迫的非特异性反应。在鱼保持在10和30摄氏度长达10天，由于丝氨酸蛋白酶和氨肽酶和溶血活性的表皮蛋白水解活性在2倍的值范围内变化。其他蛋白水解活性，由于组织蛋白酶B和L，在鱼在30摄氏度增加了长达8天，分别为3.4和2.9倍，在鱼保持在10摄氏度。这伴随着溶菌活性增加3.0倍。通过将鱼浸入柱状黄杆菌悬浮液中或给予它们腹膜内注射迟缓爱德华氏菌超过72小时，对鱼施加其他形式的应激。虽然暴露于F.感染组组织蛋白酶B和L活性在48 h内比初始值增加1.5倍以上，溶菌活性沿着增加4.5倍。暴露于E.迟到。这些结果表明，表皮组织蛋白酶B和L可能参与与日本鳗鱼皮肤的细菌溶解，它们的活动引起的环境刺激，可能是一个重要的非特异性反应的鳗鱼。
Nonspecific responses of Japanese eels to environmental stress were monitored by assaying various lytic activities in eel epidermal extract. In fish maintained at 10 and 30 degrees C for up to 10 days, epidermal proteolytic activities due to serine protease and aminopeptidase and hemolytic activity varied within a 2-fold value range. Other proteolytic activities, due to cathepsins B and L, in the fish at 30 degrees C increased for up to 8 days and were 3.4 and 2.9-fold over those in fish maintained at 10 degrees C, respectively. This was accompanied by a 3.0-fold increase in bacteriolytic activity. Other forms of stress were exerted on the fishes by immersing them in a suspension of Flavobacterium columnare or giving them intraperitoneal injections of Edwardsiella tarda over 72 h. Although serine protease and aminopeptidase activities and hemolytic activity in the fishes exposed to F. columnare changed marginally, and were similar to those in the control fish, cathepsins B and L activities in the infected fishes increased more than 1.5-fold over their initial values over a 48 h period, along with a 4.5-fold increase in bacteriolytic activity. No marked change was detected in any of the lytic activities of the fishes exposed to E. tarda. These findings indicate that epidermal cathepsins B and L probably participate in bacteriolysis associated with Japanese eel skin and that their activities are elicited by environmental stimuli and may be an important nonspecific response of eels.