Translational machinery of channel catfish: I. A transcriptomic approach to the analysis of 32 40S ribosomal protein genes and their expression
Translational machinery of channel catfish: I. A transcriptomic approach to the analysis of 32 40S ribosomal protein genes and their expression
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DOI：
10.1016/s0378-1119(02)00595-4
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发表时间：
2002-05-29
期刊：
影响因子：
3.5
通讯作者：
Liu, ZJ
中科院分区：
文献类型：
--
作者：
Karsi, A;Patterson, A;Liu, ZJ
核糖体蛋白（RP）基因已被广泛用作系统发育研究和比较基因组学的标记。然而，虽然硬骨鱼是脊椎动物中最大的一组，有超过23，000个物种，但它们还没有用于鱼类的进化研究。使用转录组学的方法，我们已经克隆和测序了32个40 S RP互补DNA（cDNA）从癍点叉尾鮰（Ictalurus punctatus），使他们的一个最完整的40 S RP基因序列从一个单一的生物体。大多数40 S RP在癍点叉尾鮰是高度相似的，他们的直系同源物在哺乳动物物种，但S19，S21和S25是高度分歧。除S26和S27基因在癍点叉尾鮰中有两个cDNA外，其余所有RP基因都只有一种cDNA。32个40S RP基因在不同组织中的表达量分别为5.33%和11.42%。RP基因的表达水平是高度可变的，在一个单一的组织之间的不同RP基因和组织之间关于一个单一的RP基因。两者合计，这些数据强烈建议RP基因表达的转录后调控，特别是在考虑到它们在核糖体中的代表的化学计量。(C)2002 Elsevier Science B.V.保留所有权利。
Ribosomal protein (RP) genes have become widely used as markers for phylogenetic studies and comparative genomics. However, they have not been available for evolutionary studies in fish although teleosts are the largest group of vertebrates with more than 23,000 species. Using a transcriptomic approach, we have cloned and sequenced 32 40S RP complementary DNAs (cDNAs) from channel catfish (Ictalurus punctatus), making them one of the most complete sets of 40S RP gene sequences from a single organism. Most 40S RPs in channel catfish are highly similar to their orthologues in mammalian species, but S19, S21, and S25 are highly divergent. Only one type of cDNA was found for all RP genes except S26 and S27, for which two cDNAs were found in channel catfish. Alternatively spliced transcripts for the S3 and alternatively polyadenylated transcripts for S 19 and S21 were found. The 32 40S RP genes are generally highly expressed and together they account for 5.33similar to11.42% of expression depending on the tissues. Expression levels of the RP genes were highly variable both within a single tissue among different RP genes and among tissues with regard to a single RP gene. Taken together, these data strongly suggest post-transcriptional regulation of RP gene expression, particularly in consideration of the stoichiometry of their representation in ribosomes. (C) 2002 Elsevier Science B.V. All rights reserved.