Vascular endothelial growth factor and basic fibroblast growth factor in children with cyanotic congenital heart disease
Vascular endothelial growth factor and basic fibroblast growth factor in children with cyanotic congenital heart disease
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DOI：
10.1016/s0022-5223(00)70133-4
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发表时间：
2000-03-01
影响因子：
6
通讯作者：
Lupinetti, FM
中科院分区：
文献类型：
--
作者：
Starnes, SL;Duncan, BW;Lupinetti, FM
目的：血管内皮生长因子和碱性成纤维细胞生长因子是血管生成的有效刺激物。患有紫绀型先天性心脏病的儿童通常会出现广泛的侧支血管形成，这可能是血管生成异常的一种形式。我们进行了本研究，以确定患有紫绀型先天性心脏病的儿童是否有血清血管内皮生长因子和碱性成纤维细胞生长因子水平升高。方法：对22例紫绀型先天性心脏病患儿和19例无紫绀型先天性心脏病患儿在心导管置入术中抽取血清。样本取自上腔静脉，下腔静脉和全身动脉。通过酶联免疫吸附法测定这些部位的血清中血管内皮生长因子和碱性成纤维细胞生长因子的水平。结果：青紫型先天性心脏病患儿血管内皮生长因子在上腔静脉（P = 0.04）和全身动脉（P = 0.02）中显著升高，而在下腔静脉中无明显升高（P = 0.2）。平均血管内皮生长因子水平，通过对所有3个部位的平均值平均测定，也显著升高（P = .03）。碱性成纤维细胞生长因子仅在全身动脉中显著升高（P = 0.02）。结论：青紫型先天性心脏病患儿血管内皮生长因子水平升高。这些发现提示这些儿童侧支血管的广泛形成可能是由血管内皮生长因子介导的。
Objective: Vascular endothelial growth factor and basic fibroblast growth factor are potent stimulators of angiogenesis. children with cyanotic congenital heart disease often experience the development of widespread formation of collateral blood vessels, which may represent a form of abnormal angiogenesis. We undertook the present study to determine whether children with cyanotic congenital heart disease have elevated serum levels of vascular endothelial growth factor and basic fibroblast growth factor. Methods: Serum was obtained from 22 children with cyanotic congenital heart disease and 19 children with acyanotic heart disease during cardiac catheterization. Samples mere taken from the superior vena cava, inferior vena cava, and a systemic artery. Vascular endothelial growth factor and basic fibroblast growth factor levels were measured in the serum from each of these sites by enzyme-linked immunosorbent assay. Results: Vascular endothelial growth factor was significantly elevated in the superior vena cava (P = .04) and systemic artery (P = .02) but not in the inferior vena cava (P = .2) of children with cyanotic congenital heart disease compared to children with acyanotic heart disease. The mean vascular endothelial growth factor level, determined by averaging the means of all 3 sites, was also significantly elevated (P = .03). Basic fibroblast growth factor was only significantly elevated in the systemic artery (P =.02). Conclusion: Children with cyanotic congenital heart disease hare elevated systemic levels of vascular endothelial growth factor. These findings suggest that the widespread formation of collateral vessels in these children may be mediated by vascular endothelial growth factor.