Effects of serotonin depletion on the play of juvenile rats
Effects of serotonin depletion on the play of juvenile rats
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DOI：
10.1111/j.1749-6632.1997.tb51942.x
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发表时间：
1997-01-01
期刊：
影响因子：
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通讯作者：
Panksepp, J
中科院分区：
文献类型：
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作者：
Knutson, B;Panksepp, J
这种打闹游戏为年幼的哺乳动物提供了在亲和环境中测试社交技能的机会。由于5-羟色胺（5-HT）的缺乏可以在成年大鼠和灵长类动物中诱导非亲和或公开的敌对行为，我们进行了一项实验，以检查5-羟色胺的缺乏是否也会增加幼年大鼠游戏行为的对抗性。（第三脑室）注射7.5 μ l的75 μ l的5，7二羟色胺（一种血清素神经毒素），混合在含有0.1%抗坏血酸的0.9%盐水溶液中。26只大鼠的对照组接受假损伤和载体注射。所有大鼠在手术前30分钟接受地昔帕明（25 mg/kg ip），在手术前20分钟接受诺米芬辛（25 mg/kg ip），以保护去甲肾上腺素能和多巴胺能神经元免受损伤。在第二次损伤处理后10天，将大鼠匹配到三种类型的游戏对中的一种：（1）假手术+假手术（Sham），（2）假手术+5 HT损伤（Mixed），或（3）5 HT损伤+5 HT损伤（5 HT-损伤）。在每天5分钟的游戏过程中（别针和背部接触），每个合作伙伴的游戏行为进行了记录，其中跨越8天。在前三个游戏环节中，被钉得最多的伙伴被认为是该对的“主导”伙伴。
Rough-and-tumble play presents opportunities for young mammals to test social skills in an affiliative context. Because serotonin (5HT) depletion can induce nonaffiliative or openly hostile behavior in adult rats’ and primates,’we conducted an experiment to examine if serotonin depletion would also increase the agonistic nature of play behavior in juvenile rats.At both 16 and 23 days of age, 26 rats received serotonin lesions via central (third ventricle) injections of 7.5 p, l of 75 p, g of 5, 7 dihydroxytryptamine (a serotonin neurotoxin) mixed in 0.9% saline solution with 0.1% ascorbic acid. A control group of 26 rats received sham lesions and injections of vehicle. All rats received desiprimine (25 mgkg ip) 30 minutes before surgery and nomifensine (25 mgkg ip) 20 minutes before surgery to spare noradrenergic and dopaminergic neurons from damage. Ten days after the second lesion treatment, rats were matched into one of three types of play pairs:(1) sham+ sham (Sham),(2) sham+ 5HT lesioned (Mixed), or (3) 5HT lesioned+ 5HT lesioned (5HT-lesioned). Play behaviors for each partner were recorded during daily 5-minute play sessions (pins and dorsal contacts), which spanned 8 days. The partner who pinned most during the first three play sessions was deemed the “dominant” partner for that pair.