Cutting edge: Immune stimulation by neisserial porins is toll-like receptor 2 and MyD88 dependent
Cutting edge: Immune stimulation by neisserial porins is toll-like receptor 2 and MyD88 dependent
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DOI：
10.4049/jimmunol.168.4.1533
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发表时间：
2002-02-15
影响因子：
4.4
通讯作者：
Wetzler, LM
中科院分区：
文献类型：
--
作者：
Massari, P;Henneke, P;Wetzler, LM
奈瑟菌孔蛋白是致病性奈瑟菌的主要外膜蛋白，其免疫增强活性是通过其刺激 B 细胞和上调 B7-2 表面表达的能力介导的。这种能力依赖于 MyD88 和 Toll 样受体 (TLR)2 的表达，MyD88 或 TLR2 敲除小鼠的 B 细胞对孔蛋白缺乏反应就证明了这一点。使用先前描述的 TLR2 依赖性报告构建体，这些结果得到证实，并表明这是由于 NF-kappaB 核易位的诱导。这是已知疫苗佐剂通过 TLR2 刺激免疫细胞的首次演示。
The immunopotentiating activity of neisserial porins, the major outer membrane protein of the pathogenic Neisseria, is mediated by its ability to stimulate B cells and up-regulate the surface expression of B7-2. This ability is dependent on MyD88 and Toll-like receptor (TLR)2 expression, as demonstrated by a lack of a response by B cells from MyD88 or TLR2 knockout mice to the porins. Using previously described TLR2-dependent reporter constructs, these results were confirmed and were shown to be due to induction of NF-kappaB nuclear translocation. This is the first demonstration of known vaccine adjuvant to stimulate immune cells via TLR2.